Scientific Program Schedule, 14th International Symposium for Immunology of Reproduction, 22-24 May, Varna, Bulgaria

Scientific Program Schedule
14th International Symposium for Immunology of Reproduction,
22-24 May, Varna, Bulgaria

Friday – May 22

08:30 h – Registration
14:00 h – Opening
          Welcome addresses to the Symposium
            Prof. Rayna Georgieva, President of ICCIR
         Prof. Guy-Andre Voisin

Session I
Immune regulation at maternal-fetal interface in implantation and pregnancy. Immunological problems of infertility, pregnancy loss, implantation failures and ART. Mucosal immunity.
Chairpersons: Guy-Andre Voison, Carolyn Coulam, Kenneth Beaman, Rayna Georgieva
• Association between immunologic causes and superfertility among women experiencing recurrent pregnancy loss
Carolyn Coulam (USA)
• Pregnancy is a model for tumors; not transplantation
Kenneth Beaman (USA)
• T  Regs and eutherian pregnancy: reconsidering  the Medawar paradigm
Gerard Chaouat (France)
• The phenotype of regulatory T (Treg) cells in normal pregnancy, miscarriage and preeclampsia.
Shigeru Saito (Japan)
• Effector Functions of Anti-HIV Antibodies in the Female Genital Tract
Jiri Mestecky (USA)

17:00 h – Coffee break
17:30 h – Session II
Innate and adaptive immunity in reproduction. Immune regulation at maternal-fetal interface in implantation and pregnancy.
Chairpersons: Nelson Fernández, Eytan Barnea, Raina Fichorova
• PIF Regulates Immune Response in vitro and in vivo by Targeting Insulin Degrading Enzyme (IDE) and Kv1.3b Proteins
Eytan Barnea (USA)
• Regulation of the major histocompatibility complex class I molecules on trophoblast cells: role of the preimplantation factor (PIF*)
Nelson Fernández (UK)
• MHC in egg-sperm interaction: evolutionary aspects
Irene Athanassakis (Greece)
• The vaginal microbiome and innate immunity in reproduction
Raina Fichorova (USA)

SOP
• The subpopulation analysis of peripheral blood lymphocytes for women with recurrent pregnancy loss after paternal lymphocyte immunotherapy
Krechetova L.V. (Russia)

20:00 h – Welcome reception

Saturday – 23 May

9:00 h – Session III
Immune regulation at maternal-fetal interface in implantation and pregnancy.
Immunological problem of infertility, pregnancy loss, implantation failures, ART.
Sexually transmitted diseases and local infections.
Chairpersons: Julia Szekeres-Bartho, UdoMarkert, Marie-Pierre Piccinni  
• Successful implantation from the embryonic view
Julia Szekeres-Bartho (Hungary)
• Placental MicroRNAs
UdoR. Markert (Germany)
• Decidual CD4+ Th17 cells are not deleterious for human pregnancy when they produce IL-4
Marie-Pierre Piccinni (Italy)
• Determinants of mother-to-infant transmission of primate lentiviruses.
Guido Silvestri (USA)
• Influence of natural regulatory T cells on pregnancy development
Velislava Terzieva (Bulgaria)

SOP
• Semen TGF-β1 and IL-18 as predictors of IVF/ICSI outcome after the intravaginal application of seminal plasma during ovum pick-up
Marina Nikolaeva (Russia)

10:30 h – Coffee break
11:00 h – Session IV
Immunological problem of infertility, pregnancy loss, implantation failures, ART.
Immune regulation at maternal-fetal interface in implantation and pregnancy.
Innate and adaptive immune mechanisms in reproduction.
Chairpersons: Joanne Kwak-Kim, Roumen Roussev, Masayuki Shimada
• Immune-regulatory role of vitamin D in autoimmune and cellular immune aspects of women with recurrent pregnancy losses
Joanne Kwak-Kim (USA)
• The dramatic changes cumulus cell functions during ovulation process impact meiotic progression and fertilization ability of oocyte
Masayuki Shimada (Japan)
• PIF, a major actor for pregnancy
François Vialard (France)
• Immunomodulatory role of low dose IVIg and Intralipid on regulatory T-cells, activated T-cells, and elevated NK populations and activity.
Roumen Roussev (Bulgaria)
•  Deciphering the Origin and the Roles of Fetal Microchimeric Cells: Work in Progress and Perspectives
Peter Sedlmayr (Austria)

SOP
• Human Decidual Stromal Cells: The low immunogenicity counterpart from foeto-maternal interface
Kameliya Vinketova (Bulgaria)
• Analysis of natural regulatory t-cells in women with reproductive failure
Andrey Velichkov (Bulgaria)

13:30 h – Lunch
14:30 h – Session V
Gametes antigenicity and gametes interactions. Innate and adaptive immunity in reproduction.  
Chairpersons: Maciej Kurpisz,  Hans Donat,  Soren Hayrabedyan
• Immunomodulatory elements in male human gonad – cytokine micropattern and biomarker genes in spermatogenic arrests – microarray study
Maciej Kurpisz (Poland)
• Role of TLR4 and NOD1 signaling in Sertoli cell innate immunity in regard to male infertility. Sertoli inflammasome and beyond.
Soren Hayrabedyan (Bulgaria)
• Fertility preservation by ovarian cryopreservation and subsequent in vitro culture of preantral follicles
Akiko Hasegawa (Japan)
• The role of cell receptors for the treatment of breast cancer
Hans Donat (Germany)
• Impact of Endocrine Disruptors on Male Reproductive and Epigenetic Parameters in a Trans-Generational Study of Mice
Jana Peknicova (Czech Republic)

17:00 h – Coffee break
17:30 h – Session VI
Immunological problem of infertility, pregnancy loss, implantation failures, ART.  Autoimmunity.
Chairpersons: Viktor Chernyshov, Tatsuo Yamamoto,  Florina Haimovici, Milena Mourdjeva
• Multiple immune deviations and IVF failure
Viktor Chernyshov (Ukraine)
• Effects of anti-angiotensin II type 1 receptor antibody and anti-β2 GPI antibody in preeclampsia
Tatsuo Yamamoto (Japan)
•  Memory T-cells in pregnancy
Natalya Sotnikova (Russia)
• Acrosin Inhibitors and their Regulation by the Ubiquitin–Proteasome System in Boar Reproductive Tract
Vera Jonáková (Czech Republic)
• Cytokines With Key Roles in Reproduction Modulated by Psychopathology
Florina Haimovici (USA)

SOP
• Vacuum-induced local intra-dermal intravascular stases modulate NK cell numbers and functions.
Boris Donskoi (Ukraine)

20:30 h – Official dinner and awarding of medals

Sunday – 24 May

9:00 h – Session VII
Immune regulation at maternal-fetal interface in implantation and pregnancy.
Immune-endocrine interactions in reproduction.  Immunological problem of infertility, pregnancy loss, implantation failures, ART. 
Chairpersons: Gil Mor, Tanya Dimova, Zdenka Ulcova – Gallova
• Placental Interferon Beta as an Immune Modulator during pregnancy
Gil Mor (USA)
• The viewpoint of the fetus: Pre-programmed to survive instead to escape
Tanya Dimova (Bulgaria)
• Long-lasting Hormonal Contraception and Antiphospholipid Antibodies
Zdenka Ulcova – Gallova (Czech Republic)
• Synergy of Infectious agents and Immunological factors in sub-fertility
 Vassilis Tsilivakos(Greece)
• Macrophages subsets in the endometrium of women with uterine leiomyoma 
Yulia Antsiferova  (Russia)
• Biomarkers of male infertility
Nina Atanassova (Bulgaria)

10:30 h – Coffee break
11:00 h – Poster Sessions I, II
13:30 h – Closing of the Symposium and granting Prof. Hans Donat award for the best posters of young scientists
14:00 h – Lunch
15:30 h – Short trip and dinner

Poster Sessions

President: Hans Donat

POSTER SESSION I (PI-1 – PI-26)
Immune regulation at maternal-fetal interface in implantation and pregnancy. Immunological problem of infertility, pregnancy loss, implantation failures, ART. Stem cells in reproduction.
Chairpersons: Julia Szekeres-Bartho, UdoMarkert, Raina Fichorova, Krassimira Todorova, Roumen Roussev

PI-1     PreImplantation Factor (PIF) Promotes Human Trophoblast Invasion
Hadia Moindjie, Esther Dos Santos, Laurence Loeuillet, Eytan R. Barnea, François Vialard, Marie-Noëlle Dieudonne.
PI-2     TGFβ1 expression at materno-fetal interface during epitheliochorial non- invasive implantation in pigs
Dimova T., R. Georgieva
PI-3     Potentials of human decidual stromal cells crosstalk with T cells
Vinketova K, Karaguozov I, Alova E, Mourdjeva M, Oreshkova Ts.
PI-4     GnRH analogues Ganirelix and Triptorelin do not influence the in vitro decidualization capacity of human endometrial stromal cells
Bochev I., Belemezova K., Shterev A., Kyurkchiev S.
PI-5     Ex-vivo screening of peripheral blood leukocytes reactivity status in pregnancy
Petkova B, Vinketova K, Alova E, Jekov V, Mincheva P, Mourdjeva M, Oreshkova Ts
PI-6     Advantages of human sperm DNA damage detection by the method of Comet Assay
Milena Georgieva, Plamen Todorov, Yosif Dimitrov, George Miloshev
PI-7     Does a genital mycoplasmas infection increase sperm DNA fragmentation in infertile men?
Rilcheva V., Pachkova S., Konova E.
PI-8     Microsphere-based flow cytometry technique for detection of blocking factors in sera from RPL patients
Gabriel Elmadjian, M. Mincheva, B.Tzenov, R. Roussev
PI-9     Prevalence of antiphospholipid antibodies other than antibodies to cardiolipin and β2-glycoprotein-I in women with recurrent pregnancy loss and their diagnostic value
Menzhinskaya I.V., Vanko L.V., Krechetova L.V., Kashentseva M.M., Sukhikh G.T.
PI-10   Anti-fibrillin-1 antibodies in RPL compared with healthy female and male individuals
M. Atanasova, E. Konova, R. Roussev
PI-11   Clinical significance of anti-78kDa glucose regulated protein (GRP78) antibodies in women with ovarian factor of infertility
I. Vangelov, V. Pesheva, G. Nikolov, M. Ivanova
PI-12   Immunological study in patients included in a program for assisted reproduction
Dimitrova-Dikanarova D. K., Damyanova V., Vatev Il.
PI-13   Can we improve pregnancy and implantation rate in cases with non-selective embryo transfer when only 1 or 2 embryos are available?
Antonova I., Bochev I., Milachich T., Valkova L., Yunakova M., Andreeva P., Shterev A.
PI-14   Analysis of the expression of CD57 in patients with reproductive failure
Antonova I.,Velichkov A., Susurkova R., Manoylov I, Nikolov G., Terzieva V.
PI-15   Efficacy of pre-pregnancy alloimmunization in treatment of recurrent pregnancy loss: time-to-pregnancy in relation to phenotype of allogenous lymphocytes and to Th1/Th2 balance in peripheral blood autogenous lymphocytes
Stepanova E.O., Nikolaeva M.A., Golubeva E.L., Vanko L.V., Krechetova L.V., Tetruashvili N.K., Sukhikh G.T.
PI-16 Gamma9Delta2 Tcells in women with recurrent spontaneous miscarriages and Chlamydia infection.
Voskakis I., Keramitsoglou Th., Avdeliodi K., Deligeoroglou E., Creatsas G., 1Varla-Leftherioti M.;
PI-17   miR-204 driven epigenetic regulation of androgen receptor
KrassimiraTodorova, Soren Hayrabedyan
PI-18   Molecular Profile And Clinicopathological Characteristics Of Prostate Cancer In Bulgarian Probands
A. Tsvetkova, A. Todorova; T. Todorov; G. Georgiev; I. Drandarska; V. Mitev
PI-19   Correlation of PIBF expression with the breast cancer grading.
Siyana Petrova, Despina Pupaki, Dessislava Ankova, Svitlana Bachurska, Rosen Panov, Dimitar Metodiev, Pavel Rashev, Milena Mourdjeva
PI-20   Localization of component renin-angiotensin system (Mas1 AND CD10) in human breast carcinoma
Dessislava Ankova, Despina Pupaki, Dimitar Metodiev, Hans Donat, Pavel Rashev
PI-21   Immunohistochemical identification of myoepithelial cells in canine mammary gland tumors
Despina Pupaki, Dimitar Metodiev, Desislava Ankova, Pavel Rashev
PI-22   Diverse effects of MSC on tumour cell lines proliferation.
Milena Kostadinova, Boris Antonov, Tsvetelina Oreshkova, Milena Mourdjeva
PI-23   Notch and SDF-1 cytokine signalling in development and reproduction
Dimova I1, Hlushchuk R, Rizov M, Penchev V, Nikolov G, Makanya A, Djonov V
PI-24   Effect of different cryopreservation methods on hematopoietic stem cells
Marina Hristova, Elena Hristova, Nadya Petrova, Plamen Todorov.
PI-25   Expression of pluripotent state-specific markers in Wharton’s jelly- derived mesenchymal stem cells
S. Kestendjieva, E. Stoyanova, L. Dzerov, A. Nikolov, T. Oreshkova, M. Mourdjeva
PI-26   Valproic and Ascorbic Acid Enhance Generation of Fibroblast-Derived iPSC
E. Stoyanova, M. Mourdjeva, S. Kyurkchiev

POSTER SESSION II (PII-27 – PII-50)
Gametes antigenicity and gametes interactions. Innate and adaptive immunity in reproduction. Metabolite and endocrine factors in reproduction.
Chairpersons: Viktor Chernyshov, Peter Sedlmayr, Velislava Terzieva, Tsvetelina Oreshkova, Tanya Dimova

PII-27             Are NETs a principal cause of seminal hyperviscosity? DNase I treatment improves sperm motility and morphology.
Angelos Gritzapis, Effrosyni Nosi, Konstantinos Makarounis, Georgios Georgoulias, Vasilios Kapetanios, Kostas Sfakianoudis, Christodoulos Papanikopoulos, Anastasia Konstantinidou, Vassilis Tsilivakos.
PII-28             Genomic and immunological abnormalities in patients with idiopathic severe male infertility  
Damyanova V., Dimitrova D. , Hadjidekova S., Dimova I., Savov A., Rukova B., Nesheva D., Vatev I., Toncheva D.
PII-29             First work for clinical verification of a systemic inflammatory response in preeclampsia
Stoeff S., Jovtchev Sv., Bechev B., Trifonova N., Buteva I., Dikov I., Vretenarska M. , Mladenova A.
PII-30 Oxidase activity of blood cells in preterm newborns with intrauterine infection
Belyaeva A.S., Balashova E.N., Vanko L.V., Matveeva N.K., Ionov O.V., Krechetova L.V.
PII-31 The impact of high scrotal temperature on Hsp70, Bcl-2, Bax and Pro-caspase-3 genes expression in the reproductive organs of sexually mature mice
S. Zapryanova, P. Rashev, T. Dimova, Y. Martinova, M.Mollova
PII-32 Differences in seminal plasma proteins between dogs with good and poor quality of the sperm
Gradinarska Desislava, Ivanova-Kicheva Maria, Daskalova Denica, Hristova Marina, Valeva Yanitsa, Lazov Kiril
PII-33 Possible Function of Glyceraldehyde-3-phosphate Dehydrogenase-spermatogenic Protein GAPDHS in Mammalian Sperm
Hasmik Margaryan, Andriy Dorosh, Jana Capkova, Pavla Manaskova-Postlerova, Anatoly Philimonenko, Pavel Hozak, Jana Peknicova
PII-34 Morphofunctional characteristic of frozen-thawed semen from Danube horse breed
Z Shekerov, G Nenkova, S Okoliyski, M Chervenkov, P Taushanova, A Aleksandrova, R Stefanov, B Georgiev
PII-35 Identification of seminal plasma proteins related to low cryotolerance of boar spermatozoa
Daskalova Denica, Kirilova Irina,  Hristova Marina, Gradinarska Desislava, Lazov Kiril, Ivanova-Kicheva Maria.
PII-36 Role of Brain-Derived Neurotrophic Factor (BDNF) and its receptor TrkB in reproductive system in developing rats: possible role for Leydig cell and germ cell development
Yochkova S, Lakova L, Pavlova E, Dimova D, Atanassova A
PII-37 Hormonal changes of functional and pathological impotence during male andropause
Vencislav Milkov, Kremena Miteva, Ganka Pironcheva, Kiril Lazov, Teodora Daneva, Volodia Georgiev
PII-38 Amount of energy in the daily meal and reproductive characteristics of cows
Stojanovski  M., Tomovska J., Joshevska E.
PII-39 Ralationships between plasma melatonin, cortisol and LH  in male buffaloes
P. Taushanova, S. Manchev, L. Kanchev and P. Tzvetkova
PII-40 Ovulation control and pregnancy rate in Danube breed mares inseminated with frozen semen
Z Shekerov, M Chervenkov, P Taushanova, B Georgiev, Maria Elena Falomo, G. Yordanov, S.Okoliyski
PII-41 Chromatography (HPLC) profile of ram seminal plasma proteins and their role on the quality parameters of the spermatozoa
Valeva Yanitsa, Ivanova-Kicheva Maria, Gradinarska Desislava, Hristova Marina, Lazov Kiril, Daskalova Denica
PII-42 Estrogen receptor beta (ERβ) in reproductive tract of male mice
Fatima Elzeinova, Pavla Dostalova, Lukas Ded, Andriy Dorosh, Jana Peknicova
PII-43 Immunolocalization of estrogen receptor α in rabbit ovary
Abadjieva D., Ankova D., Rashev P., Kistanova E.
PII-44 Decrease of  PR/ER ratio in endometrial cancer versus uterine myomas in post-menopausal women
Vencislav Milkov, Kremena Miteva, Ganka Pironcheva, Kiril Lazov, Teodora Daneva, Volodia Georgiev
PII-45 Effect of okadaic acid treatment on cytoskeletal structures and the chromosome spread in in vitro maturing mouse oocyte
V. Hadzhinesheva, V.  Nikolova, I. Chakarova, S. Delimitreva, M. Markova, R. Zhivkov
PII-46 Dynamics of intermediate filament proteins in mouse oocytes from germinal vesicle to metaphase II stages
V. Nikolova, M. Markova, R. Zhivkova, R. Dimitrov, V. Hadzhinesheva, I. Chakarova, S. Delimitreva
PII-47 Influence of genetic background on oocyte meiotic spindle, actin cap and chromosomes in inbred mice
I. Chakarova, V. Nikolova, R. Zhivkova, M. Markova, V. Hadzhinesheva, T. Tenev, S. Delimitreva
PII-48 Immunocytochemical study of ovarian hormone receptors and growth factors in non-human primate (Macaca Fascicularis)
Kamihigashi M, Hasegawa A, Kato T, Morimoto N, Sankai T, Shibahara H
PII-49 Profiling of gene expression during early folliculogenesis in mice
Sugiyama,Y, Hasegawa A, Takeyama R, Morimoto A, Kumamoto K, Shibahara H
PII-50 Synchronization of estrus cycle in Danube horse mares
Z Shekerov, M Chervenkov, P Taushanova, B Georgiev , Maria Elena Falomo ,
G. Yordanov, S.Okoliyski