Immunoneuroendocrynology

  • Main research topics

    Head of Department– Rossitza KonakchievaPhD, DSc, Professor

    Research topics

    • Male and female neuroendocrine control of reproductive performance. Influence of stress and environmental factors on endocrine and immune mechanisms of subfertility.
    • Chronobiology and neuroendocrine mechanisms of adaptation. Relationship between pineal gland and adrenocortical axis. Immunological aspects and physiological relevance.
    • Homeostatic neuroendocrine regulation of the immune reactivity: regulatory interactions between neuroendocrine and immune systems.
    • Scientific achievements

      Most relevant scientific achievements

      • Studies on the pineal hormone melatonin and its relevance as a circadian regulator of physiological functions have revealed role in neuroendocrine-immune homeostasis through control on glucocorticoid secretion in supreme brain centers and through direct inhibitory action on lymphocyte proliferation. The direct action of melatonin in primary and secondary immune tissue has been confirmed by several methods including radioligand-receptor analysis, autoradiography, Western and immune blot analysis, ICH and ISHH. The established biochemical characteristics correspond to those found for the trans-membrane G-protein coupled receptor (Mel 1А) in neural tissue. Distinct diurnal variations in the binding capacity of the receptors in rat spleen and thymus have been found which most probably reflect the chronomodulating action of the hormone on the functions of immunocompetent T-lymphocytes. Increased endogenous levels of glucocorticoids due to immobilization stress participated in modulation of the receptor characteristics for melatonin in secondary immune tissue, sensitized with T-dependent antigen.
      • In longitudinal studies of patients with disbalanced glucocorticosteroid and hypophysiotropic secretion (Cushing’s syndrome), recruited at the Center of Endocrinology, MU, Sofia, several endocrine and immune parameters have been correlated to circadian melatonin pattern in peripheral blood and saliva. Except for the disturbances in the gonadotropin secretion (luteinizing, follicle-stimulating hormones and prolactin), we found significant differences of clinical relevance concerning chronobiological parameters of the endogenous rhythm of melatonin and cortisol (acrophase, amplitude, % rhythm) associated to different etiology of the disease. We were able to show that the abnormal encreased activity and the absence of regular circadian rhythm of the glucocorticoid axis are accompanied by disturbances in the rhythm of melatonin secretion and the presence of altered pro-inflammatory immune response.
      • In collaboration with the Laboratory of Endocrinology and Metabolism, Albert Einstein College, New York, the modulation role of thiazolidininedions (insulin-sensitizing pharmaceutical agents, agonists of PPAR-gamma) on the signal pathways of insulin and expression of StAR protein in mixed ovarian cultures has been investigated. A model for interaction with potential significance in the physiological and the pathological response to insulin has been proposed. The action of insulin and TZDs mediated by their receptors results in stimulation of the StAR protein in ovary tissue and activate IRS-1 – a key component of the insulin signal pathway. These results contribute to the clarification of the therapeutic role of insulin-sensitizing agents in relation to reproductive problems with metabolic component in women.
      • The role of chronobiological controlled factors of antioxidant protection against highly reactive oxidation radicals, especially in male reproductive system, and their importance in the etiology of idiopatic male infertility has been studied. Obtained pilot results indicate that melatonin has relevance to the fertilization capacity of human spermatozoa, most probably through modulation of the balance of growth factors /EGF, testosterone, esradiol/ and enzymes /aromatase, NOS-synthase/ in semen plasma.
      • The Radioisotope laboratory for hormonal diagnostics at the Department of Immunoneuroendocrinology is performing complex radioimmunological and radioligand-receptor analyses of peptide and non-peptide hormones and biologically active compounds. By using high sensitive direct RIA methods, in-vitro models for examination of cultivated tissues and cells from female and male reproductive tissue have been established and validated, which allow investigation of reproductive characteristics at cellular level. Pilot studies on the cryopreservation of human ovary tissue based on the use of these models have been awarded in 2003 with Signum Laudis pro Sciencae Merriti of Medical University of Sofia for contribution to the clinical medical science.
      • Research grants

        RESEARCH CONTRACTS

        National Science Fund Contract 1614/ 2006 Studies on the antioxidant potential of melatonin for improvement of reproduction capacity in idiopatic infertile men. Principal Investigator: Assoc. Prof. Rossitza Konakchieva, DSc.

        National Science Fund Call Promotion of Scientific Research in Governmental Universities 2010. New three-dimensional in-vivo – like cell culture system for studies on fibroblast differentiation. Contract granted by between Sofia University, Institute of Biophysics and IBIR. Principal Investigator from IBIR – Assoc. Prof. Rossitza Konakchieva, DSc.

        INTERNATIONAL RESEARCH GRANTS

        Thanks to Scandinavia Foundation 2004 – 2008. Mechanism of action of insulin-sensitizing agents in human ovary. Principal Investigator – Research Associate Dimiter Avtanski.

        Gerald & Dorothy Friedman Foundation 2004-2008.Role of PPAR –gamma in human ovary– Principal Investigator – Research Associate Dimiter Avtanski.

        Contract for scientific extramural collaboration with University of Scopje, Macedonia 2005 – Endocrine mechanisms of adaptation in domestic animals. Principal Investigator from IBIR – Assoc. Prof. Rossitza Konakchieva, DSc.

        Contract for scientific extramural collaboration with University of Stara Zagora 2005 –Influence of biological active substances on endocrine parameters and natural adaptation capacity. Assoc. Prof. Rossitza Konakchieva, DSc.

        • Publications

          Scientific papers published during last 5 years.

          Papers in International peer-reviewed scientific journals

          I.Ilieva, P.Tzvetkova, I.Sainova, P.Taushanova, M.Kacarov, E.Zvetkova    Immature spermatogenic cells in semen fluids of infertile men. Compt.Rend.Acad.Bulg.Sci., 69, 1, 85-94, 2016.
          ISSN: 1310-1331    IF: 0.284

          S.Manchev, R.Stefanov, G.Anev, P.Cvetkova, N.Maksimovic Effect of melatonin on the reproductive characteristics of rams of north east Bulgarian fine fleece breed. Compt.Rend.Acad.Bulg.Sci., 67, 12, 1701-1706, 2014.
          ISSN: 1310-1331 IF: 0.284

          P.D.Cvetkova, I.N.Ilieva Polyorchidism and fertility. MD-Medical Data, 6, 1, 29-32, 2014.
          ISSN: 1821-1585

          P.Tzvetkova, I.Ilieva, S.Rangelov, I.Babyuk, S.Shamraev Changes in the secretion of gonadothropins LH, FSH and infertility. Compt.Rend.Acad.Bulg.Sci., 66, 3, 459-466, 2013.
          ISSN: 1310-1331         IF: 0.198

          I.Ilieva, S.Ivanova, S.Rangelov, P.Tzvetkova, V.Ormandzhieva, E.Petrova Sperm changes in cases with Varicocele. Compt.Rend.Acad.Bulg.Sci., 66, 5, 757-764, 2013.
          ISSN: 1310-1331 IF: 0.198

          E.Vachkova, B.Bivolarski, M.Gulubova, R.Konakchieva, I.P.Georgiev, R.Simeonov Immunohistochemical localization of EGF receptors in the intestinal tract of growing rabbits in relation to age. Livestock Science, V.142, 216-221, 2011.
          ISSN: 1871-1413;       IF – 1.506

          R.Konakchieva, P.Todorov Melatonin protects human spermatozoa from apoptosis via melatonin receptor- and extracellular signal-regulated kinase-mediated pathways. Letter to Editor. Fertility and Sterility, V.96, No 4 e159, 2011.
          ISSN: 0015-0282;      IF – 3.958

          Papers in National (Bulgarian) scientific journals

          П.Цветкова, П.Таушанова, С.Манчев FSH и фертилитет. Андрология, 24, 3, 10-12, 2015.
          ISSN: 1310-3806

          П.Цветкова, П.Таушанова, И.Костадинова, С.Манчев Тестостерон и мъжка репродуктивна патология. Уронет, 1, 9 – 16, 2015.
          ISSN:1312-1960

          P.Tzvetkova, I.Ilieva, M.Kacarov, E.Zvetkova, J.Chitalov, Iv.Dechev Immature spermatogenic cells in semen fluids of infertile men. Андрология, 24, 4, 2-5, 2015.
          ISSN: 1310-3806

          I.Ilieva, P.Tzvetkova, M.Kacarov, I.Sainova, P.Taushanova, I.Vladov, E.Zvetkova Round Immature Spermatogenetic Cells in Semen Fluids of Infertile Men with Diagnosis “Migrating Testis”. Two Casuistic Cases in Adults. Acta Morphologica et Anthropologica, 22, 15-23, 2015.
          ISSN: 0861-0509

          Ю.Караиванова Влияние на тежките метали върху репродукцията на организма. Андрология, 24, 4, 15-20, 2015.
          ISSN: 1310-3806

          И.Костадинова Микрочастици в мъжкия урогенитален тракт. Андрология, 24, 1, 12-14, 2015.
          ISSN: 1013-3806

          С.Манчев, П.Таушанова, И.Костадинова Физиологична роля ма мелатонина и инфертилитет. Андрология, 23, 2, 6-11, 2014.
          ISSN: 1013-3806

          П.Таушанова, Ю.Караиванова, П.Цветкова Биохимична характеристика на семенната плазма и нейното клинично значение. Андрология, 23, 2014, 4, 16-19.
          ISSN: 1310-3806

          П.Таушанова Глюкокортикоид – зависима модулация и мелатонин. Андрология, 23, 3, 17-19, 2014.
          ISSN: 1310-3806

          П.Таушанова Ефект на мелатонин в in vitro модели на антиген/митоген стимулирана пролиферация на клетъчни култури от човешки лимфоцити. Андрология, 23, 2014, 4, 7-10.
          ISSN: 1310-3806

          П.Таушанова Участие на епифизния хормон мелатонин в имунорегулацията. Андрология, 23, 3, 7-10, 2014.
          ISSN: 1310-3806

          И.Илиева, П.Цветкова Антиоксидантна защита на сперматозоидите при оксидативен стрес. Андрология, 23, 1, 14-20, 2014.
          ISSN: 1013-3806

          П.Цветкова Клинично значение на подвижността на сперматозоидите. Андрология, 23, 2, 16-18, 2014
          ISSN: 1013-3806

          П.Цветкова, Й.Узунова, С.Рангелов, И.Бабюк, С.Шамряев Морфологични нарушения в тестикуларната тъкан при прекъсната сперматогенеза ха ниво сперматиди. Урология, 20, 1, 22-27, 2014
          ISSN: 1310-6058

          И.Костадинова Експресия и физиологична роля на ароматаза в мъжки полови клетки. Андрология, 23, 3, 11-19, 2014.
          ISSN: 1310-3806

          Р.Конакчиева Имуноневроендокринология – приемственост и развитие. Списание на БАН, 127, 1, 27-30, 2014.
          ISSN: 0007-3989

          И.Илиева, П.Цветкова Влияние на оксидативния стрес върху сперматогенезата, спермазоидите и патология на мъжката гюлова система. Андрология, 22, 4, 12-20, 2013.
          ISSN: 1310-3806

          В.Георгиев, П.Цветкова Геномна активност при сперматогенезата. Андрология, 22,
          3,16-20,2013.
          ISSN: 1310-3806

          Рангелов С, П. Цветкова Кои са причините за мъжкия инфертилитет? Андрология, 22, 1,4-10,2013.
          ISSN: 1310-3806

          П.Цветкова, В.Георгиев Спермиацията – процес на освобождаване на зрели сперматиди в лумена на семенните каналчета. Андрология, 22, 2, 9-15, 2013.
          ISSN: 1310-3806

           

          Popular Science, Current Affairs publications and Educational publications
          Books and brochures

          1. Цветкова П. Медико-биологични аспекти на мъжкия инфертилитет и тяхното диагностично значение. Научна Фондация “Андрология”, София, 2013, 258 с.
          ISBN: 987-954-92357-3-9

          G. Georgiev, J. Pastuschek, S. Neubeck, U. Markert Part I: Substances secreted by the preimplantation human embryo. Chapter 6 in Immunology of Pregnancy – 2013. ed. G. Chaouat, S. Olivier, N. Ledee; pp. 384-396, Bentham Science Publishers, 2013.
          ISBN: 978-1-60805-734-4, eISBN: 978-1-60805-733-7

           

          Previous publications

          Papers in National (Bulgarian) scientific journals

          1. Todorov P, Konakchieva R, Dimitrov J, Chub N, Petrenko Y. Vitrification of human embryonic stem cells for cryopreservation. Comptes rendus de l’Academie bulgare des Sciences 2005, 58, 9:1095-1100 ISSN: 1310-1331.

          2.Todorov P., Konakchieva R.,Dimitrov J. Effect of recombinant FSH on the synthesis and secretion of estradiol 17-beta from human granulosa cells in-vitro. Endocrinologia, 2005, X, 3:149-153 ISSN 1310-8131 .

          3Todorov P., Konakchieva R., Dimitrov J., Slavchev B., Novachkov W. Cryopreservation of human ovarian tissue-our experience. Embryologia 2006, 1: 4-8 ISSN 1312-7349.

          4. Автански Д. Действие на тиазолидиндиони върху стероидогенезата в човешки яйчник: ин витро изследване. Ендокринология 2006; XI, 3:170-177 ISSN 1310-8131.

          5.Georgiev G., Konakchieva R. Epigenetic modeling of the embryonal and the postnatal development. Embryologia. 2007, 2, 1:16-20 ISSN 1312-7349.

          6.Gjorgovska N, Filev K, Konakchieva R. Influence of induced molting on hormonal status of aged laying hens. Krmiva (Zagreb) 2008; 1; 19-25 ISSN: 0023-4850.

          Papers in International peer-reviewed scientific journals

          7. Seto-Young D, Paliou M, Schlosser J, Avtanski D, Park A, Patel P, Holcomb K, Chang P, Poretsky L Direct thiazolidine action in the human ovary: insulin-independent and insulin-sensitizing effects on steroidogenesis and insulin-like growth factor binding protein-1 production. J Clin Endocrinol Metab 90(11):6099-6105, 2005 ISSN: 0021-972X (IF=5.8).

          8.Seto-Young D, Avtanski D, Strizhevsky M, Parikh G, Patel P, Kaplun J, Holcomb K, Rosenwaks Z, Poretsky L.Interactions among peroxisome proliferator activated receptor-γ, insulin signaling pathways and steroidogenic acute regulatory protein in human ovarian cells.J Clin Endocrinol Metab 92(6):2232-9, 2007 ISSN: 0021-972X (IF=5.8).

          9. Таushanova P., Georgiev G, Manchev S, Konakchieva R. Effect of glucocorticoids on melatonin receptor expression under T-cell activated immune response. Comptes Rendus de l, Academie des Sciences, 60:899-902, 2007 ISSN: 1310-1331 (IF=0.106).

          10. Stephanova E., Topouzova-Hristova T., Konakchieva R. Mitochondria are involved in stress response of A549 alveolar cells to halothane toxicity.Toxicology In Vitro; 22(3):688-694, 2008 ISSN 0887-2333 (IF=2.05).

          11. Tomova A., Kumanov P., Robeva R., Manchev S., Konakchieva R. Melatonin secretion and non-specific immune responses are differentially expressed in corticotropin-dependent and corticotropin-independent Cushing’s syndrome. Medical Science Monitor; 14(6):CR327-332, 2008 ISSN (electronic) 1643-3750 (IF= 1.59).

          12. Todorov P., Hristova E., Konakchieva R., Michova A., Dimitrov Y. Comparative studies of different cryopreservation methods for mesenchymal stem cells derived from human fetal liver. Cell Biology International; 34:455-4622010.ISSN 1065-6995 (IF=1.62)

          13. Vachkova E, Penchev I, Georgiev B, Biviloarski B, Konakchieva R. Relationships between plasma concentrations of Epidermal Growth Factor,            insulin and iodated thyroid hormones in early andnormal weaned rabbits. Revue Méd. Vét. 161, 1, 30-36, 2010 ISSN: 0035-1555 (IF=0.09).

          14.Todorov P., Konakchieva R, Dimitrov Y., Novachkov V., Kyurkchiev D., Tchoob N., Gorohova N. Morphological and functional assessment of             human fetal     liver cells in long-term culture. Problems of Cryobiology 2006, 2: 201-206 ISSN: 0233-7673.

          15.Dineva J., Vangelov I., Nikolov G., Konakchieva R., Ivanova M. Nitric oxide stimulates the production of atrial natriuretic peptide and progesterone byhuman granulose luteinized cells with an antiapoptotic effect. Endocrine Regulations; 42 (2-3):45-51, 2008 ISSN (electronic): 1336-0329.

           

          1. Seto-Young D, Paliou M, Schlosser J, Avtanski D, Park A, Patel P, Holcomb K, Chang P, Poretsky L Direct thiazolidine action in the human ovary: insulin-independent and insulin-sensitizing effects on steroidogenesis and insulin-like growth factor binding protein-1 production. J Clin Endocrinol Metab 90(11):6099-6105, 2005 (IF=5.8).
          2. Seto-Young D, Avtanski D, Strizhevsky M, Parikh G, Patel P, Kaplun J, Holcomb K, Rosenwaks Z, Poretsky L. Interactions among peroxisome proliferator activated receptor-γ, insulin signaling pathways and steroidogenic acute regulatory protein in human ovarian cells. J Clin Endocrinol Metab 92(6):2232-9, 2007 (IF=5.8).
          3. Таushanova P., Georgiev G, Manchev S, Konakchieva R. Effect of glucocorticoids on melatonin receptor expression under T-cell activated immune response. Comptes Rendus de l, Academie des Sciences, 60:899-902, 2007 (IF=0.106).
          4. Stephanova E., Topouzova-Hristova T., Konakchieva R. Mitochondria are involved in stress response of A549 alveolar cells to halothane toxicity. Toxicology In Vitro; 22(3):688-694, 2008 (IF=2.05).
          5. Tomova A., Kumanov P., Robeva R., Manchev S., Konakchieva R. Melatonin secretion and non-specific immune responses are differentially expressed in corticotropin-dependent and corticotropin-independent Cushing’s syndrome. Medical Science Monitor; 14(6):CR327-332, 2008 (IF= 1.59).
          6. Todorov P., Hristova E., Konakchieva R., Michova A., Dimitrov Y. Comparative studies of different cryopreservation methods for mesenchymal stem cells derived from human fetal liver. Cell Biology International; 34:455-462, 2010 (IF=1.62)
          7. Vachkova E, Penchev I, Georgiev B, Biviloarski B, Konakchieva R. Relationships between plasma concentrations of Epidermal Growth Factor,     insulin and iodated thyroid hormones in early andnormal weaned rabbits.     Revue Méd. Vét. 161, 1, 30-36, 2010 (IF=0.09).
          8. Todorov P., Konakchieva R, Dimitrov Y., Novachkov V., Kyurkchiev D., Tchoob N., Gorohova N. Morphological and functional assessment of     human fetal     liver cells in long-term culture. Problems of Cryobiology 2006, 2: 201-206.
          9. Dineva J., Vangelov I., Nikolov G., Konakchieva R., Ivanova M. Nitric oxide stimulates the production of atrial natriuretic peptide and progesterone byhuman granulose luteinized cells with an antiapoptotic effect. Endocrine Regulations; 42 (2-3):45-51, 2008.
          10. Seto-Young D, Avtanski D, Parikh G, Suwandhi P, Strizhevsky M, Araki T, Rosenwaks Z, Poretsky L. Rosiglitazone and pioglitazone inhibit estrogen synthesis in human granulosa cells by interfering with androgen binding to aromatase. Horm Metab Res 43:1-8, 2011 [article in press]

          Publications in national journals without IF
          1. Todorov P, Konakchieva R, Dimitrov J, Chub N, Petrenko Y. Vitrification of human embryonic stem cells for cryopreservation. Comptes rendus de l’Academie bulgare des Sciences 2005, 58, 9:1095-1100.
          2. Todorov P., Konakchieva R., Dimitrov J. Effect of recombinant FSH on the synthesis and secretion of estradiol 17-beta from human granulosa cells in-vitro. Endocrinologia, 2005, X, 3:149-153.
          3. Todorov P., Konakchieva R., Dimitrov J., Slavchev B., Novachkov W. Cryopreservation of human ovarian tissue–our experience. Embryologia 2006, 1: 4-8.
          4. Автански Д. Действие на тиазолидиндиони върху стероидогенезата в човешки яйчник: ин витро изследване. Ендокринология 2006; XI, 3:170-177
          5. Georgiev G., Konakchieva R. Epigenetic modeling of the embryonal and the postnatal development. Embryologia. 2007, 2, 1:16-20.
          6. Gjorgovska N, Filev K, Konakchieva R. Influence of induced molting on hormonal status of aged laying hens. Krmiva (Zagreb) 2008; 1; 19-25.

          PARTICIPATION IN SYMPOSIA
          1. Seto-Young D, Paliou M, Schlosser J, Patel P, Park A, Avtanski D, Latif W, Babar N, Yeshou D, Omry G, Holcomb K, Poretsky L Peroxysome     proliferator-activated receptor g (PPARg) in human ovarian cells: its role in regulation of steroidogenesis and IGFBP-1 production The Endocrine Society’s 87th AnnualMeeting, San Diego, CA, USA, Abstract P3-564 , 2005.
          2. Dineva J., Vangelov I., Konakchieva R., Stefanova Ts., Ivanova M. „ Studies on the immunoendocrine regulation of cell survival and apoptosis in preovulatory human follicle”, 5th European Congress of Reprod Immunol., Aug.-Sep. 2007.
          3. Dineva J., Vangelov I, G. Nikolov, R. Konakchieva, Ts. Stefanova, M. Ivanova Study on the survival role of ANP-cGMP-P and NO in human periovulatory follicle. X International congress of reproductive immunology, Opatija, Croatia,     June 10-14, 2007.
          4. Konakchieva R, Manchev S, Tomova A, Kumanov P. Neuroendocrine and immune correlates of impaired hypothalamo-pituitary-adrenal function. Exp Clin Endocrinol Diabetes 2005 9th Annual Meeting Deutsche Neuroendokrinologie Group, Dresden Germany.
          5. Динева Ж., Вангелов И., Конакчиева Р, Николов Г, Иванова М, „Апоптоза на гранулоза лутеинни (GLCs) и кумулусни клетки (CCs) свързана с възрастта на жени в програма за IVF”. Шести Национален Конгрес по Стерилитет, Контрацепция и Хормонозаместителна терапия, Боровец, 13-16 Март 2005
          6. Аvtanski D., D. Seto-Young, R. Konakchieva, L. Poretsky Insulin- independent and insulin-sensitizing effects of thiazolidinediones in human ovary. 11th Int. Symp. of Immunology of Reproduction, Varna, 2-4 June 2006.
          7. Dineva J., I. Vangelov, G. Nikolov, R. Konakchieva, J. Martinova, M. Ivanova In vitro study on the role of epidermal growth factor, progesterone and cyclc guonosine monophosphate on granuslose cells survival. 11th Int. Symp. of Immunology of Reproduction, Varna, 2-4 June 2006.
          8. J. Dineva, I. Vangelov, G. Nikolov, R. Konakchieva, M. Ivanova Comparative studies of some parametters for success in patients treated with GnRHa (Decapeptyl) and GnRHant (Orgalutran) from assisted reproduction program. 8th National Congress of Infertility, Contraception and Hormone-replacement therapy and Gynaecological Endoscopy with international participation, Borovetz, 17-19 of March 2007.
          9. J. Dineva, I. Vangelov, G. Nikolov, R. Konakchieva, Ts. Stefanova, M. Ivanova Study on the survival role of ANP-cGMP-P and NO in human periovulatory follicle. X International congress of reproductive immunology, Opatija, Croatia, June 10-14, 2007; American Journal of Reproductive Immunology 57 (2007), 379-481, ICRI 07-1-31
          10. J. Dineva, I. Vangelov, R. Konakchieva, Ts. Stefanova, M. Ivanova Studies on the immunoendocrine regulation of cell survival and apoptosis in preovulatory human follice. XIIIth Symposium of Czech reproductive immunologists with international participation, Zdar nad Sazavou, 24-27 May, 2007. American Journal of Reproductive Immunology 58 (2007), 181-237, SOP 19
          11. I. Wawrek, R. Konakchieva. 3rd EMBIC Summer School, Jena, Germany 2007. POS 39.
          12. J.Dineva, I.Vangelov, R.Konakchieva, Ts.Stefanova, M.Ivanova, “Studies on the local immunoendocrine regulation of cell survival in preovulatory human follicle” V European congress of Reproductive immunology, Berlin, 3-7 september, 2007 Journal of Reproductive Immunology 81 (2009), pp.152
          13. V. Ivanova, I. Wawrek, R. Konakchieva Influence of endocrine and paracrine factors on the functional characteristics of spermatozoa of men with idiopathic infertility. 9th National Congress of Infertility, Contraception, HRP and Gynaecological Endoscopy with International Participation, 11-14 April, 2008.
          14. G. Georgiev, I. Wawrek, R. Konakchieva Effects of growth factor and glucocorticoids on the steroidogenic response of human granulose cells in vitro. 9th National Congress of Infertility, Contraception, HRP and Gynaecological Endoscopy with International Participation, 11-14 April, 2008.
          15. Dineva J, Vangelov I, Nikolov G, Gulenova D, R. Konakchieva     Stefanova Tz, Ivanova M. The atrial natriuretic peptide- cGMP- progesterone     and nitric oxide as a pro- survival factors for human granulosa luteinized cells. 12th International Symposium of Immunology of Reproduction, ISIR June 25- 27, Varna, Bulgaria, 2009
          16. MD Ivanova, J. Dineva, I. Vangelov, R. Konakchieva, V. Terzieva. Studies on the local immunoendocrine regulation of cell survival in preovulatory human follicle. 7th European Congress on Reproductive Immunology. September 17-20, 2009 Marathon, Greece.
          17. MD Ivanova, J. Dineva, I. Vangelov, R. Konakchieva, V. Terzieva. Studies on the local immunoendocrine regulation of cell survival in preovulatory human follicle. 12th International Symposium of Immunology of Reproduction, ISIR June 25- 27, Varna, Bulgaria, 2009
          18. Avtanski D, Strizhevsky M, Parikh G, Araki T, Rosen O, Demetri C, Goldman M, Cadag S, Rosenwaks Z, Poretsky L, Seto-Young DThe effects of thiazolidinediones on estrogen production in human granulosa cells The Endocrine Society’s 89th Annual Meeting, Toronto, Canada, Abstract P1-317, 2007
          19. Avtanski D, Park A, Kaplun J, Strizhevsky M, Kantor Y, Holcomb K, Poretsky L, Seto-Young DEffects of mitogen-activated protein kinase (MAPK) inhibition on progesterone and insulin-like growth factor binding protein-1 (IGFBP-1) production in human ovarian cells The Endocrine Society’s 88th Annual Meeting, Boston, MA, USA, Abstract P1-409, 2006
          20. Avtanski D, Kaplun J, Strizhevsky M, Park A, Patel P, Kantor Y, Kearny Brown M, Dhillon S, Pang X, Goldman M, Yeshou D, Moosavy A, Holcomb K, Rosenwaks Z, Seto-Young D, Poretsky L Interactions among PPARg, insulin signaling pathways and aromatase in human ovarian cells The Endocrine Society’s 88th Annual Meeting, Boston, MA, USA, 2006 Abstract P1-397.
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