Immunobiology of Reproduction

Main research topics

Head of department – Assoc. prof. Pavel Istiliynov Rashev

Basic research topics

  • Factors and mechanisms controlling cell proliferation, differentiation and apoptosis in male and female reproductive systems.
  • Identification, characterization and functional activity of receptors and ligand molecules participating in gametes interaction.
  • Immune-endocrine regulation of the ovarian function. Biological and clinical significance of autoimmunity against ovarian antigens.
  • Local regulatory factors and mechanisms in structural remodeling of cellular immunity during the phases of implantation and pregnancy.
  • Development of model systems and methods for the study of the fertilizing capability of gametes in vitro, the diagnosis and treatment of immunologically mediated infertility in humans and animals.
  • Role of metalloproteinases in normal breast physiology and in breast pathology.

Scientific achievements

Most important scientific achievements (last 5 years)

  • Sperm specific, membrane-bound antigens expressed in various animal species including humans have been identified and characterized using monoclonal and polyclonal antibodies produced by us: antigen 1F10 has shown receptor characteristics; antigen 4B12 – appeared as an adhesive molecule; antigen 3G4 has role in the piruvate – lactate cellular metabolism and antigen 60 participate in functional steps required for sperm-egg fusion process. The antifertile effect of antibodies coresponding to these antigens showed their relevance to the fertilization process and their possible significance for immunocontraception.
  • An increased expression of Hsp70 and αB-Cry stress proteins detected using immunohistochemical, immunochemical and genetic studies have been demonstrated in testes of adult mice under conditions of heat-induced apoptosis. Hsp70 is synthesized in germ cells at all stages of spermatogenesis cycle while the synthesis of αB-Cry starts in puberty during differentiation of germ cells in the pachytene stage of meiotic prophase. These studies are important for elucidating the role of stress proteins during proliferation, differentiation and apoptosis of germ cells and their possible role in modulating the fertile potential of the germ cells.
  • It was found that the incidence of auto-antibodies against Zona Pellucida (ZP) and Granuloza luteinized cells (AGA) in sera and follicular fluids of infertile women were negatively related to the IVF outcome. The frequency and the level were higher in infertile compared to fertile women.
  • It was demonstrated that in the preovulatory follicle the gonadotropin induced system of Atrial natriuretic peptide – cyclic Guanosine Monophosphate – Progesterone exists and shares a regulatory role in the mechanisms of apoptosis mediating the expression of anti-apoptotic bcl-2 and inhibiting executory caspase-3 activity as well as the expression of pro-apoptotic p53. The rate of expression of immunoreactive Atrial natriuretic peptide (ANP) in Granulosa luteinized cells (GLCs) corresponded to its concentration in respective follicular fluids and in supernatants of cultured GLCs as evidence that these cells are the main source of ovarian ANP in the preovulatory human follicle
  • It was demonstrated that the level of apoptosis in human Granulosa luteinized cells (GLCs) increases with the age in infertile women and is higher in mural GLCs than in Cumulus cells (CCs). The rate of apoptosis in CCs is directly related to the maturity of oocytes. Apoptosis of mural GLSs and CCs may be the potential marker for the hormonal stimulation and the In vitro fertilization outcome in women undergoing assisted reproduction
  • It was proven that during the implantation and early pregnancy of the pig the trophectoderm and endometrial epithelium express the integrins (a5β1, avβ3, av, β3), ECM proteins (fibronectin, vitronectin, laminin, collagen type IV), and MMPs-2,9 in an implantation-time dependent manner, which gave evidence for the molecular events participating in the structural remodeling and establishment of the epitheliochorial placenta.
  • Original data for the presence of T-cell aggregates into endometrial compartments during implantation, named T-cell clusters (TCC), were obtained. Both phenotype and localization of TCC are implantation-phase dependent. Endometrial total T, cytotoxic T, and helper T cells, measured by flow cytometry, are highest in number at the critical phase of the implantation – the time of the attachment of genetically different tissues – trophectoderm and maternal epithelium.
  • It was proven by RT-PCR that during the implantation endometrial immune cells are potent to produce the immunomodulatory Th3 cytokine TGFβ1, a finding supporting the hypothesis about Th3-mediated bystander suppression during implantation.
  • It could be suggested that i) the marked Т-cell response and presence of TCC, ii) the decrease and specific re-distribution of NK cells far from placenta attachment site and iii) the production of immunomodulatory substances during the superficial implantation in pigs are possible “pieces” of the complex mechanism of the maternal immune tolerance towards to the semialogeneic fetus.
  • It has been proved that Са2+ bound protein Secretagogin plays an important role in the synthesis and secretion of insulin and that dexamethazone as a member of the glucocorticoid family disturbs the insulin secretion through influence on the synthesis, the intracellular distribution and secretion of Secretagogin.

Research Projects

Research Projects for the last 5 years:

I. Bulgarian Academy of Science proprietary Budget subsidized:

1. Project Title: “Characterization of sperm antigens with fusogenic activity in fertilization” 2007-2009
PI: Associate Professor Dr. Margarita Mollova

2. Project Title: “Expression and characterization of matrix metalloproteinases with a role in the processes of spermatogenesis and fertilization, 2008-2009
PI: Assistant Professor Dr Pavel Rashev

3. Project Title: “Studies on antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) levels in follicular fluid of infertile patients and their relationship to the outcome of assisted reproduction. Relationship between SOD and vitality of human granulosa lutein cells (GLCs)”, 2009-2010
PI: Associate Professor Dr Maria E. Ivanova

4. Project Title: “Immuno-endocrine regulation of ovary”, 2010-2011
PI: Assistant Professor Ivaylo Vangelov

5. Project Title: “Regulatory mechanisms in luteinisation and apoptosis in preovulatory follicle”, 2010-2011
PI: Assistant Professor Dr Julieta Dineva

6. Project Title: “Non-invasive test of the genetic potential of cumulus cells as biomarkers for selecting oocytes in program for in vitro fertilization”, 2010-2011.
PI: Assistant Professor Dr Krassimira Todorova-Hayrabedyan

II. Projects funded by NSF

1. Project MU-K1 1306/03 “Identification and characterization of sperm molecules fusogenic activity in the interaction of gametes and their significance for fertility”, 2004 – 2007, 4500 EUR
PI: Assistant Professor Dr Julieta Dineva

2. Project Contract № L 1407/04 “Role of regulatory T cells during HIV-1 infection and immunological recovery during HAART”, 2004-2008, 15 000 lv.
PI – Associate Professor Dr Velislava Terzieva

3. Project Contract № L 1505/05 “Evaluation of the immunogenicity of peptides from the RD1 region of M. tuberculosis in individuals with active or latent tuberculosis infection immunized with BCG vaccine”, 2005-2010, 15 000 lv.
Participant IBIR – Associate Professor Dr Velislava Terzieva

4. Project B 1537/2005 “Local cellular and molecular mechanisms involved in the induction of immune tolerance during pregnancy in epitheliochorial type placenta”, 2006-2009, 26500 lv.
PI: Professor Dr Raina Georgieva, DSc

5. Project B1517/05 “The Importance of calcium-binding protein Secretagogin in hormone-secreting neuroendocrine cells”, 2006-2010. 20600 lv.
PI: Assistant Professor Teodora Daneva

6. Project B 1507/2005 “Regulatory mechanisms in luteinization and apoptosis in preovulatory follicle”, 2005-2009. 25400 lv.
PI: Associate Professor Dr Maria E. Ivanova

7. Project MU-B/1503/2005 “Characterization of antigens associated with ovarian autoimmunity”, 2006-2009. 9900 lv.
PI: Assistant Professor Dr Ivaylo Vangelov

8. Project TKB-1606 “Localization and function of stress proteins in testicular germ cells in heat-induced and spontaneous apoptosis”, 2007 – 2010., 42.000 Euro
PI: Associate Professor Dr. Margarita Mollova

9. Project DOO2-172/08 “New temperature-sensitive superoxide dismutase from Antarctic fungi: biotechnological study, chemical characterization and application” – a Joint project with the Institute of Microbiology-BAS, 2009-2011, IBIR – 19.530 Euro for the first 18 months.
PI (IBIR) – Associate Professor Dr. Margarita Mollova

10. Project DOO2-92/15.04.2009 “Preparation of research projects for the 7th Framework Programme of EU”, 2009-2010, 6.000 EUR
PI: Associate Professor Dr. Margarita Mollova

11. Project DOO2-50/2008 “Building Scientific Research Centre on the problems in reproductive health” under Program “Development of scientific infrastructure”, 2009- 2011, 800 000 lv.
PI: Associate Professor Dr. Margarita Mollova

III. Additional projects funded by contracts ministries, departments and companies in the country

1. Project “Impact of anti-ovarian antibodies on fertility,” concluded with: Medical Centre ReproBioMed, 2010-2011. (application and commercialization of scientific products)
2. Project “Impact of anti-ovarian antibodies on fertility,” concluded with: Centre for Reproductive Health “Hope”, 2010-2011. (application and commercialization of scientific products)
3. Project “Impact of anti-ovarian antibodies on fertility,” concluded with: Medical Centre “Reproductive health” 2007-2011. (application and commercialization of scientific products)
4. Project “Impact of anti-ovarian antibodies on fertility,” concluded with: Medical Centre “Faith”, 2007-2011. (application and commercialization of scientific products)
5. Project “Studies on the gene regulation of Mas1, ACE2 and ACE in normal and pathological tissue from patients with breast cancer” – concluded with the private Gynaecology Clinic “Professor Hans Donat”, Magdeburg (partner organization IBIR project under 7th EU FP) and private Hospital “Tokuda”, Sofia, 2010-2011.
PI: Associate Professor Dr Pavel Rashev

IV. Additional projects funded contracts and programs of the EU, NATO, UNESCO and others. International Organizations

1. PROJECT TO CALL FP7-REGPOT-2009-1 (ReProForce) “Enhancing scientific capacity of the Bulgarian Institute of Biology and Immunology of Reproduction, 2009-2013, EUR 1,500,000
Project Coordinator: Associate Professor Dr. Margarita Mollova

2. PROJECT TO CALL FP7-PEOPLE-2009-IEF “Effects of IL-7 and IFNa on the development of nTreg”, concluded between Bulgaria (IBIR) and France (INSERM), 2011-2013
Participant (Bulgaria): Associate Professor Dr Velislava Terzieva

3. Participation in Project: BG051PO001/07/3.3-02/33 funded by the Operative Programme “Human Resources” of the European Social Fund “Training of PhD students in basic research skills – a prerequisite for a dynamic career in accordance with the needs of the Labour Market”, 2008 – 2010
PI: Raya Staykova, Centre for Science Studies and History of Science.
Participants (Bulgaria): Ph.D. Konstantin Danailov

4. Participation in Project: BG051PO001/07/3.3-02/33 funded by the Operational Programme “Human Resources” of the European Social Fund – “Expanding the skills and qualifications of young scientists – a prerequisite for professional development in border areas between science and practice” 2010 – 2011.
PI: Raya Staykova, Centre for Science Studies and History of Science.
Participants (Bulgaria): Silvina Zapryanova and Alexander Kukov

V. EBR and projects within the institute-to-institute collaboration

1. Project “Role of spermatozoa selected proteins in fertilization,” with the Institute of Genetics, Czech Academy of Sciences, 2003-2006.
PI from IBIR: Associate Professor Dr. Margarita Mollova
2. Project “Studies on the control mechanisms of differentiation, luteinisation and apoptosis in ovarian cells” with the Polish Academy of Sciences, Jagiellonian University, Krakow: Institute of Zoology, 2006 – 2009.
PI from IBIR: Associate Professor Dr. M. D. Ivanova
3. Project “Expression of genes and proteins with a role in reproduction – biomarkers for infringements” Institute of Genetics, Czech Academy of Sciences, 2007 – 2010.
PI from IBIR: Associate Professor Dr. Margarita Mollova
4. Project “Expression of genes and proteins with role in reproduction” with the Institute of Biotechnology, Czech Academy of Sciences, 2011 – 2013.
PI from IBIR: Associate Professor Dr. Margarita Mollova

Scientific publications

Scientific papers published during last 5 years.

Papers in International peer-reviewed scientific journals

Dimova T, M. Brouwer, F. Gosselin, J. Tassignon, O. Leo, C. Donner, A. Marchant, D. Vermijlen. Effector Vg9Vd2 T cells dominate the human fetal γδ T cell repertoire. Proceedings of the National Academy of Sciences USA, 112, 6, 2015.
ISSN:1091-6490 IF:9.809

D.Pupaki, D.Ankova, V.Vasilev, P.Rashev   Localization of MMP-2 and MMP-9 in canine mammary gland during pregnancy. Journal of Biomedical and Clinical Research, 7, 2, 99-104, 2014.
ISSN 1313-6917

S.A.Yotov, A.S.Atanasov, G.B.Georgiev, J.D.Dineva, N.A.Palova   Investigation on some biochemical parameters and effect of hormonal treatment in anoestrous dairy cows with cystic ovarian follicle. Asian Pacific J.of Reproduction, 3, 1, 41-45, 2014.
ISSN: 2305-0500 (Print) ISSN: 2305-0519 (Online)

S.Zaprjanova, P.Rashev, D.Zasheva, Y.Martinova, M.Mollova   Electrophoretic and immunocytochemical analysis of Hsp72 and Hsp73 expression in heat-stressed mouse testis and epididymis. European J.Obstetrics & Gynecology and Reproductive Biology, 2013.
ISSN: 0301-2115         IF: 1.627

S. Yotov, A. Atanasov, J. Dineva   Influence of postpartum luteal activity on the reproductive performance in Bulgarian Murrah Buffaloes. Veterinarija ir Zootechnika (Vet Med Zoot), T. 62 (84), 20-27, 2013.
ISSN: 1392-2130        IF: 0. 101(2012)

T. Daneva, Sh. Pashova, R. Emilova, P. Padeshki, H. Gagov, V. Georgiev   DREAM regulates insulin promoter activiry through newly identified DRE element. Central European J. of Biology, 8, 2, 97-106, 2013.
eISSN: 1644-3632, print ISSN:1895-104X IF: 0.633

Lech A., Daneva T., Pashova S., Gagov H., Crayton R., Kukwa W., Czarnecka A. M., Szczylik C.   Ovarian cancer as a genetic disease. Front. Biosci., 18, 543-563, 2013.
ISSN (printed): 1093-9946. ISSN (electronic): 1093-4715. IF: 4. 249

Atanasov A,  Dineva J,  Yotov S.  Ultrasonic evaluation of uterine involution in Bulgarian Murrah buffalo after administration of oxytocin. Anim. Reprod. Sci, 2012, 133, 71-76, 2012.
ISSN: 0378-4320.  IF: 1,750

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova  Experimental human and rodent models for balanced activity of oncogenes and tumor-suppressor genes in Vitro and In Vivo. Journal of Scientific Research in Pharmacy, 1, 2, 38-42, 2012.
ISSN: 2277-9469

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, Sh.Pashova, E.Nikolova  (2012)  Experimental In Vitro human and animal models for balanced activity of oncogenes and tumor-suppressor genes and their protein products. Scholarly Journals of Biotechnology, 1, 1, 8-14, 2012.
ISSN: 2315-6171

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova    Experimental In Vitro Mouse and Human Stem Cell Models for Balanced Activity of Oncogenes and Tumor-Suppressor Genes. J.Phys.Pharm.Adv., 2, 1, 64-68, 2012.
ISSN: 2251-7693

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova    Experimental Rodent and Human In Vitro models for balanced activity of oncogenes and tumor-suppressor genes. J.Phys.Pharm.Adv., 2, 4, 165-170, 2012.
ISSN: 2251-7693

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova   Experimental In Vitro-Stem Cell models for balanced activity of oncogenes and tumor-suppressor genes from Mouse and Human. Advances in Bioresearch, 3, 1, 69-72, 2012.
ISSN: 0976-4585.

J.Dineva, I.Vangelov, R.Abrashev, K.Todorova, D.Gulenova, G.Nikolov, I.Rangelov, G.Stamenov, M.Angelova, M.Ivanova   Survival role of superoxide dismutase 1 on human granulose luteinized cells un vitro. Endocrine regulations, V.45, 175-181, 2011.
ISSN: 1210-0668

J.Dineva, I.Vangelov, G.Nikolov, D.Gulenova, M.Ivanova   Atrial natriuretic peptide is an antiapoptotic factor for human granulose luteinized cells with impact on the results of CON/IVF in women undergoing IVF program. Journal of Obstetrics and Gyneacology Reserch, V.37, 6, 511-519, 2011.
ISSN: 0020-7292; IF – 1,704

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova   Achievment of balanced oncogenes and tumor-suppressor genes activity in normal and malignant cells In vitro and In vivo. J. of Developmental Biology and Tissue Engineering, Vol.3 (7), 85-91, 2011.
ISSN: 2141-2251

K.Lampichler, A.Ilhan, R.Lang, T.Daneva, L.Wagner, E.Knosp, B.Niederle, S.M.Baumgartner-Parzer   Evalution of MEN1 risk individuals bearing R171Q.R171Q – single nucleotide polymorphism (SNP) or not SNP – that is the guestion. Clinical Endocrinology, 75, 274, 2011.
ISSN: 0300-0664 IF – 3.168

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova    Experimental in vitro rodent and human normal and malignant cell models for balanced activity of oncogenes and tumor-suppressor genes. J.Life Sciences, 5, 454-459, 2011.
ISSN: 1934-7391

I.Sainova, I.Vavrek, V.Pavlova, T.Daneva, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova   Experimental model for safe in vitro- and in vivo- influence of internal and external factors of cell differentiation. African J. of Pharmacy and Pharmacology, V.5 (6), 786-791, 2011.
ISSN: 1996-0816; IF – 0.839

I.Sainova, I.Vavrek, V.Pavlova, I.Iliev, L.Yosifova, E.Gardeva, E.Nikolova, T.Daneva, R.Nitsch, A.Nitsch   In vitro- and In vivo- experimental models for balanced activity of oncogenes and tumor-suppressor genes in normal and malignant cells. Clinical Reviews and Opinions, Vol. 3 (4) 37-44, 2011.
ISSN: 2141-2553

V.Gerzilov, P.Rashev, A.Bochukov, P.Bonchev  Effect of semen extenders on sperm motion of in vitro stored Muscovy drake spermatozoa. Biotechnology in Animal Husbandry, 27, 3, 733-740, 2011.
ISSN: 1450-9156

Papers in National (Bulgarian) scientific journals

Velichkov A., Susurkova R., Antonova I., Manoylov I., Nikolov G., Terzieva V.    Gender associated variations in human peripheral t regulatory cells. Comptes rendus de l’Acade ́mie bulgare des Sciences, 68, 6, 775 – 785, 2015.
ISSN: 1310-1331 IF: 0.284

Susurkova R., Kutleva Ts., Velichkov A., Mihaylova N., Antonova I., Nikolov G., Terzieva V.   Tregs population impairment is associated with an increased risk of spontaneous abortion. Annuaire de l’Universite de Sofia “St. Kliment Ohridski”, Faculte de Biologie, 100, 4, 135–143, 2015.

Georgiev, G, Ankova, D. Light microscopic and immunohistochemical study of the medial collateral ligament epiligament in rat knee. Comptes rendus de l’Acade ́mie bulgare des Sciences, 68, 1, 95-100, 2015.
ISSN: 1310-1331 IF: 0.284

V.Terzieva, J.Dutrieux, V.Fabre-Mersseman, M.Rancez, B.Charmeteau-de Muylder, S.Figueiredo, R.Cheynier   Analysis of natural regulatory T-cells in HIV-1-infected patiens under therapy. Compt.Rend.Acad.Bulg.Sci., 67, 7, 1011-1018, 2014.
ISSN: 1310-1331 IF: 0.284

П.Рашев   Имунобиология на репродукцията – фундаментални и приложни изследвания. Списание на БАН, 127, 1, 10-13, 2014.
ISSN: 0007-3989

Д. Ташкова, С. Бачурска, Д. Стайков, Ц. Михалкова, П. Рашев, В. Беловеждов    Тъканна матрица – мост между наука и диагностика. Медицински преглед, 50, 2, 25-28, 2014.
ISSN: 1312-2193

В.Василев, Д.Пупаки, О.Йорданов   Сравнително проучване степента на токсичност (LD50) на отровата от двата подвида на пепелянката – Vipera ammodytes ammodytes и Vipera ammodytes meridionalis. Екология и бъдеще, 13, 3, 81-89, 2014.
ISSN: 1312-0751

Т.Данева, К.Митева, В.Георгиев   Експресия на RUVBL1 по време на сперматогенезата. Андрология, 23, 1, 7-10, 2014.
ISSN: 1013-3806

G.Georgiev, B.Landzhov, S.Slavchev, P.Rashev, T.Todorov, L.Malinova, L.Stokov, W.Ovtscharoff  Comparative election microscopic and immunohistochemical study of stromal cells in giant cell tumor of bone. Scripta Scientifica Medica, 45, Suppl.l, 19-22, 2013.
ISSN 0582-3250 (Print). ISSN 1314-6408 (Online)

J. Dineva, I. Vangelov. l. Rangelov. G. Stamenov, T. Fartsova   Role of leptin in oxidative stress and survival of human granulosa luteinized cells in vitro. Acta Medica Bulg, 2, 33-38, 2013.
ISSN: 0324-1750

R. Emilova, D. Dimitrova, V. Georgiev, T. Daneva, H. Gagov   Cystatione gamma-lyase as a regulator of resistance artery contraction under normal and hyperglycemic conditions. Bulgarian Journal of Agricultural science, 19, 175-177, 2013.
ISSN: 1310-0351         IF: 0.189 (2012)

K.Todorova, S.Hayrabedyan, J.Dineva, I.Vangelov, D.Zasheva, V.Penchev, G.Nikolov, M.Mollova, M.Ivanova   Cumulus biomarker evaluation for human oocyte quality prediction. Acta Medica Bulg., Vol.XXXIX, No1, 70-76, 2012.
ISSN: 0324-1750

Georgiev GP, Landzhov B, Slavchev S, Rashev P, Stokov L, Ovtscharoff W.   Localization of matrix metalloproteinase-2 in giant cell tumor of bone. Compt.Rendus Acad.Bulg.Sci., 65, 9, 1285-1288, 2012.
ISSN 1310-1331; IF–0.210

I.Vangelov, J.Dineva, K.Todorova, D.Gulenova, G.Nikolov, M.D.Ivanova  Ovarian auto-antibodies are associated with poor in vitro fertilization of oocytes. Compt.Rendus Acad.Bulg.Sci., 65, 9, 2012.
ISSN 1310-1331.  IF : 0.210

J.Dineva, I.Vangelov, K.Todorova, T.Stefanova, G.Nikolov, D.Gulenova, M.D.Ivanova  Relationship of  Nitrate levels in follicular fluids (FFls) and rate of apoptotic human granulosa luteinized cells (GLCs) with  the COH/IVF outcome. Compt.Rendus Acad.Bulg.Sci., 65, 1, 115-120, 2012.
ISSN 1310-1331.  IF : 0.210

J.Dineva, I.Vangelov, K.Todorova, R.Abrashev, D.Gulenovа, G.Nikolov, М.Аngelova, М.Ivanova   Study on the relationship between activity of antioxidant enzymes Superoxide dismutase and Catalase in granulosa luteinized cells with COH / IVF outcome in infertile women. Compt.Rendus Acad.Bulg.Sci., 65, 2, 181-186, 2012.
ISSN 1310-1331. IF : 0.210

Динева Ж., И. Вангелов, Р. Абрашев, Д. Гуленова, Г. Николов, Т. Чаушев, Г. Стаменов, М. Ангелова, М. Иванова   Ефективна антиоксидантна ензимна защита на човешки гранулоза лутеинни клетки: предпоставка за повишена клетъчна виталност и по-добри резултати при инфертилни жени в програма за IVF-ET. Репродуктивно здраве, 18, 14-22, 2011.
ISSN: 1312-6180

Динева Ж., И.Вангелов, Цв.Стефанова, Д.Гуленова, Г.Николов, М.Иванова    Изследвания върху ролята на азотния оксид (NO) в преовулаторния човешки фоликул. Репродуктивно здраве, 18, 8-13, 2011.
ISSN: 1312-6180

J.Dineva, AK Wojtowicz, K.Augustowska, I.Vangelov, K.Todorova, EL.Gregoraszczuk, M.D.Ivanova   Study on the control of apoptosis of porcine granulose cells after in vitro luteinization. 2nd  Conference of the Balkan Network for the Biotechnology in Animal Reproduction. Proceeding, Sofia, 24-25 March 2011.
ISSN: 1314-3085

S.Zaprjanova, P.Rashev, D.Zasheva, Y.Martinova, M.Mollova   Electrophoretic analysis of HSP72 and HSP73 expression in male reproductive tissues. Compt.Rendus Acad.Bulg.Sci., 64, 4, 529-534, 2011.
ISSN: 1310-1331; IF – 0.219

Лолов С.   Вентилация на средното ухо. GP News, 1, 1-5, 2011.
ISSN: 1311-4727

Лолов С. Имунологични фактори в етиопатогенезата на рецидивиращия и ексуадативен среден отит. МБ оториноларингология, 4, 29-34, 2011.
ISSN: 1312-6997

M.Mollova, P.Rashev, M.Stamenova   Immunochemical and biochemical investigations on boar sperm antigen with functional activity in primary sperm-ZP binding. 2nd  Conference of the Balkan Network for the Biotechnology in Animal Reproduction. Proceeding, Sofia, 24-25 March 2011.
ISSN: 1314-3085

M.Mollova, D.Dimitrova, K.Danailov, M.Genov, M.Stamenova, P.Rashev   Investigation on the immunogenecity of the sperm-specific antigen AG60 involved in gamete fusion in infertile patients. Compt.Rendus Bulg.Acad.Sci., 64, 1, 53-60, 2011.
ISSN: 1310-1331; IF -0.210

Mollova M., E. Kistanova   Reinforcement of the research capacity of the Bulgarian Institute of Biology and Immunology of Reproduction. Advances in Bulgarian Science, National Centre for Information and Documentation, 68-70, Sofia, 2010.
ISSN 1312-6164

Y.Martinova, M.Petrov, M.Mollova, M.D.Ivanova   Study on the structural changes of cat zona pellucida (ZP) during oocyte development and after fertilization by transmission (TEM) and scanning electron microscopy (SEM). 2nd  Conference of the Balkan Network for the Biotechnology in Animal Reproduction. Proceeding, Sofia, 24-25 March 2011.
ISSN: 1314-3085

Тодорова К., С.Хайрабедян, Ж.Динева, И.Вангелов, В.Пенчев, Г.Николов, М.Моллова, М.Иванова   Изследвания върху генетичният потенциал на кумулсните клетки като биомаркери за селекция на ооцити по програма за ин витро оплождане. Репродуктивно здраве, 18, 23-33, 2011.
ISSN: 1312-6180

K.Todorova, D.Zasheva, S.Hayrabedyan, J.Dineva, I.Georgiev, V.Penchev, G.Nikolov, M.Mollova, M.Ivanova   Gene panel in human cumulus cells as biomarker successful in vitro procedures. Compt.Rendus Bulg.Acad.Sci., 64, 8, 1143-1150, 2011.
ISSN: 1310-1331; IF – 0.219

K.Todorova, I.Georgiev, J.Dineva, V.Penchev, S.Hayrabedyan, G.Nikolov, M.Mollova, M.Ivanova   Lysil oxidase as a potential biomarker for predicting oocyte quality. Compt.Rendus Bulg.Acad.Sci., 64, 9, 1355-1362, 2011.
ISSN: 1310-1331; IF – 0.219

Popular Science, Current Affairs publications and Educational publications 

 Books and brochures

Vangelov I., J.Dineva, K.Todorova, S.Hayrabedyan, M.D.Ivanova   Ovarian biomarkers in infertility.  In:  Trends in Immunolabelled and Related Techniques, chapter 8, 101-132. Ed. E.Abuelzein, Published by InTech,  2012.

ISBN: 978-953-51-0570-1

Previous publications

Papers in International peer-reviewed scientific journals

1. Gartner W, Mineva I, Daneva T, Baumgartner-Parzer S, Niederle B, Vierhapper H, Weissel M, & Wagner L. A newly identified ret proto-oncogene polymorphism is found in a high number of endocrine tumor patients. Human Genetics 117: 143-153 (2005). ISSN: 0340-6717

2. Zierhut B, Daneva T, Gartner W, Brunnmaier B, Mineva I, Berggård T, & Wagner L. Setagin and secretagogin-r22: posttranscriptional modification products of the secretagogin gene. Biochemical and Biophysical Research Communications 329: 1193-1199 (2005).ISSN: 0006-291X

3. Mollova M., M. Ivanova, B. Atanassov, M. Petrov, St. Kyurkchiev. Study of the role of a sperm membrane-associated antigen in canine fertilization. Reproductive Biology, 5(3); 331-339 (2005). ISSN: 0031-5362, IF – 0.882

4. Rashev P, Georgieva R, Rees D. Expression of α5β1 integrin and fibronectin during early pregnancy in pigs. Folia Biologica, 51(5); 121-125 (2005). ISSN: 0015-5500, IF – 0.719

5. Vangelov I, Dineva J, Nikolov G, Lolov S, Ivanova M. Antibodies against granulosa luteinized cells and their targets in women attending IVF program. American Journal of Reproductive Immunology, 53: 1–7 (2005) ISSN: 0271-7352, IF – 2.172

6. Zierhut B, Daneva T, Gartner W, Brunnmaier B, Mineva I, Berggård T, & Wagner L. Setagin and secretagogin-r22: posttranscriptional modification products of the secretagogin gene. Biochemical and Biophysical Research Communications 329: 1193-1199 (2005).ISSN: 0006-291

7. Gartner W, Koc F, Nabokikh A, Daneva T, Niederle B, Luger A, & Wagner L. Long-term in vitro growth of human insulin-secreting insulinoma cells. Neuroendocrinology83: 123-130 (2006). ISSN: 0028-3835 IF – 2.422

8. Mollova M, B. Atanassov, R. Nedkova, St. Kyurkchiev. Biochemical and immunochemical characterization of boar sperm flagellar protein with role in hyperactivation/capacitation. Reproductive Biology, 6(1): 79-94, (2006). ISSN: 0031-5362, IF – 0.882

9. Mollova M, M. Ivanova, B. Georgiev, M. Petrov, S. Kyurkchiev. Sperm specific monoclonal antibody that affects canine gametes interaction. www. Voorlaasdagen. org, 2006.

10. Markova R., V. Terzieva, R. Drenska, Y. Todorova, E. Sapundjieva, D. Stefanova, V. Marinov, K. Kostov, I. Elenkov, M. Yankova: Evaluation of a whole blood IFN-γ assay in immunocompetent and immunocompromised Bulgarian patients with MTB infection. Clin. Applic. Immunol, 5(1):548-555 (2006) ISSN: 1312-0832

11. Dineva J., Anna K. Wójtowicz, Katarzyna Augustowska, Ivailo Vangelov, Ewa L. Gregoraszczuk, Maria D. Ivanova. Expression of Atrial Natriuretic Peptide (ANP), Progesterone (P), Apoptosis-Related Proteins (bcl-2, p53) and Caspase-3 Activity in in vitro Luteinized and Leptin-Treated Porcine Granulosa Cells (pGCs). Enodocrine Regulations, 1: 11-18 (2007) ISSN (printed): 1210-0668. ISSN (on line): 1336-0329.

12. Gartner W, Vila G, Daneva T, Nabokikh A, Koc-Saral F, Ilhan A, Majdic O, Luger A, & Wagner L. New functional aspects of the neuroendocrine marker secretagogin based on the characterization of its rat homolog. Am J Physiol Endocrinol Metab 293: 347-354 (2007). ISSN: 0193-1849. IF – 3.855

13. Rashev P. Presence of plasma and cellular fibronectin in uterine secretions of pigs during early pregnancy. Journal of Central European Agriculture, 8(1); 115-120 (2007). ISSN: 1332-9049

14. Dineva J, Vangelov I, Nikolov G. , Konakchieva R., Ivanova M. Nitric oxide (NO) stimulates the production of Atrial Natriuretic Peptide (ANP) and Progesteron (P) by human Granulosa Luteinized Cells (GLCs) with an antiapoptotic effect. Endocrine Regulations 42: 45-51, (2008 ) ISSN (printed): 1210-0668. ISSN (on line): 1336-0329.

15. Zaprjanova S, Y.Martinova P.Rashev, M. Mollova. Early ultrastuctural changes in mouse testis and epididymis after single exposure to heat. Andrologiia, 16(4), 8-10, (2007). ISSN: 1310-3806

16. Ilhan A, Gartner W, Nabokikh A, Daneva T, Majdic O, Cohen G, Böhmig GA, Base W, Hörl WH, & Wagner L. Localization and characterization of the novel protein encoded by c20orf3. Biochem J., 414: 485-495 (2008). ISSN 0264-6021. ISSN: IF – 5.155

17. Martinova Y, Petrov M, Mollova M, Rashev P, Ivanova M. Ultrastructural study of cat zona pellucida during oocyte maturation and fertilization. Animal Reproduction Sciences, 108; 425-434 (2008). ISSN: 0378-4320, IF – 1.890

18. Terzieva V. Regulatory T cells and HIV infection. Viral Immunology, 21(3): 285-292 (2008) ISSN:0882-8245 IF – 1.779

19. Markova R., Drenska R., Todorova Y., Terzieva V., Stefanova D. Monitoring the efficacy of anti-TB therapy by using the QuantiFERON-TB Gold In tube test. Eur. Resp. Rev., 17: 74-75 (2008). ISSN: 0905-9180.

20. Seghrouchni F., Contini S., Markova R., Drenska R., Sadki K., Baassi L., Todorova Y., Terzieva V., Bocchino M., Cappelli G., Altieri A. M., Alma M. G., Benjouad A., Mariani F., Petrunov B., Colizzi V., El Aouad R., Saltini C., Amicosante M. Design of immunogenic peptides from Mycobacterium tuberculosis genes expressed during macrophage infection. Tuberculosis, 210-217 (2009). ISSN: 1472-9792. IF – 2.536

21. Terzieva V., Popova D., Kitcheva M., Todorova Y., Markova R., Martinova F., Elenkov I., Yankova M. Correlation between the degree of immune activation, production of IL-2 and FOXP3 mRNA levels in CD4+CD25+ T regulatory cells in HIV-1 infected subjects under HAART. Int. Immunoph., 9: 831-836 (2009). ISSN: 15675769. IF – 2.214

22. Sainova I, Vavrek V, Pavlova S, Daneva T, Manchev S., Nikolova E. Experimental model for safe gene transfer by recombinant gene constructs. Biotechnol. & Biotechnol. 24: 134 – 138 (2010). ISSN 1310-2818.

23. Zahariev P, Sapundzhiev E, Pupaki D, Rashev P, Palov A, Todorov T. Morphological Characteristics of the Canine and Feline Stomach Mucosa. Anatomia, Histologia, Embryologia, 39(6); 563-568 (2010). ISSN: 0340-2096 IF – 0.616

24. Dineva J, Vangelov V, Nikolov G, Gulenova D, Ivanova M. Atrial natriuretic peptide is an antiapoptotic factor for human granulosa luteinized cells with impact on the results of COH/IVF in women undergoing IVF program. Journal of Obstetrics and Gynaecology Research (2011)(in press) ISSN: 1447-0756, IF- 0.777

 

Papers in National (Bulgarian) scientific journals

 1. Daneva T, Zierhut B, Gartner W, & Wagner L. Fine tuning of insulin transcription by secretagogin variants. Comp. Rend. Bulg. Acad. Sci. 8: 983-988 (2005). ISSN: 1310-1331, IF – 0.204

2. Markova R., Drenska R., Terzieva V., Todorova Y., Dimitrov V., Nikolova M.: The application of the T SPOT-TB assay in Bulgarian patients with tuberculosis infection. Probl. Infect. Paras. Dis., 33(2): 10-11 (2005)

3. Drenska R., Markova R., Terzieva V., Todorova Y., Dimitrov V., Peneva E., Nikolova M. Comparative evaluation between the QuantiFERON-TB immunodiagnostic assay and the classical methods for tuberculosis infection diagnosis. Infectology, 3: 31-34 (2005). ISSN 0861-8259

4. Vangelov I, Dineva J, Nikolov G, Genov M, Ivanova M. Anti-granulosa antibodies (AGA) in follicular fluids and their impact to controlled ovarian hyperstimulation and on the fertilization rate. Comp. Rend. Bulg. Acad. Sci. 59: 1191-96 (2006). ISSN: 1310-1331, IF – 0.204

5. Dineva J, Vangelov I, Nikolov G, Gulenova D, Ivanova M. Apoptosis of human Granulosaluteinized (GLCs) and Cumulus cells(CCs) as a possible indicator for IVF outcome. Comp. Rend. Bulg. Acad., 59: 781-784 (2006) ISSN: 1310-1331, IF – 0.204

6. Mollova M, Martinova Y. Immunocytochemical localization of antigen involved in sperm-zona pellucida interaction. Acta Morphol. et Antropol., 13, 98-101 (2006). ISSN 0861-0509

7. Terzieva V., Markova R. Drenska R., Todorova Y, Elenkov I., Yankov M, Stefanova E. Possibilities for use of QuantiFERON-TB test in patients co-infected with HIV-1 and M. Tuberculosis. Pneumology and phthisiology, 1: 9-14 (2006). ISSN 0324-1491

8. Todorova Y, Markova, R., Terzieva V, Drenska R., Kostov, K., Elenkov I, Yankov M, Stefanova E. Characterization of cell-mediated immune function in patients co-infected with HIV-1 and M. Tubecrulosis. Pneumology and phthisiology, 1: 15-20 (2006). ISSN 0324-1491

9. Drenska R., Markova R., Terzieva V., Todorova Y, Dimitrov V., Penev E., Nikolova M. Identification of ESAT-6 and CFP-10 specific T-lymphocyte epitopes in patients with active TB in the course of pathogen-specific therapy. Pneumology and phthisiology, 1: 21-23 (2006). ISSN 0324-1491

10. Terzieva V: Role of regulatory T cells in immune pathogenesis of HIV-1 infection. Infectology, 2: 3-6 (2006). ISSN 0861-8259

11. Martinova Y, Mollova M, Petrov M, Rashev P, Ivanova M. TEM and SEM characteristics and lectin binding distribution of cat zona pellucida during oocyte maturation and fertilization. Comp. Rend. Bulg. Acad. Sci. 60(7); 789-794 (2007). ISSN: 1310-1331,IF – 0.204

12. Mineva I, Stamenova M, Popovska S, Betova T, Rashev P, Sapundzhiev E, Simeonova M, Trifonova N. Localization of low molecular weight stress protein (sHSP) alpha B-crystallin in human thyreoid gland. Comp. Rend. Bulg. Acad. Sci., 60(4); 449-452 (2007). ISSN: 1310-1331, IF – 0.204

13. Mollova M, Zaprjanova S, Rashev P, Martinova Y. Developmental and hyperthermia-related expression of heat shock protein 70 (HSP 70) in mouse testis. Comp. Rend. Bulg. Acad. Sci., 60(8); 903-906 (2007). ISSN: 1310-1331, IF – 0.204

14. Zaprjanova S., Y. Martinova, P. Rashev, M. Mollova. Early ultrastructural changes in mouse testis and epididymis after single exposure to heat. Andrologiia, 16, 8-10, 2007 (in Bulgarian)

15. Mollova M. Current problems of the reproductive biology of farm animals in Bulgaria. Animal breeding sciences, 4, 4-6, 2007. ISSN 0204-7225 (in Bulgarian)

16. Stamenova M., S. Popovska, T. Betova, P. Rashev, E. Sapundzhiev, M. Simeonova, N. Trifonova. Expression of low molecular weight stress proteins α-crystallins in human placenta in different types of pathology. Embriologia, 2, 3-8, 2007 (in Bulgarian). ISSN 1312-7349.

17. Trifonova N., B. Anguelov, P. Rashev, E. Sapundzhiev, I. Buteva, M. Stamenova. Expression of eye lens antigens – alpha crystallines in extraocular human fetal tissues. Bulgarian review of ophtalmology, 1, 49-54, 2007 (in Bulgarian)

18. Markova R., Amicosante M., Drenska R., Contini S., Todorova Y., Seghrouchni F., Terzieva V., Petrunov B.: Selection of mycobacterium tuberculosis – Specific T-cell epitopes for immune diagnosis. Comptes rendus de l’Academie bulgare des Sciences, 60(2): 205-214 (2007). ISSN: 1310-1331. IF – 0.240

19. Martinova Y., S. Zaprjanova, D. Tzvetkov, M. Mollova. Immunoexpression of heat shock proteins in human cryptorchid testes. Application of modified method for immunocytochemistry on semithin sections. Comp. Rend. Acad. Bulg. Sci., 61(8), 1027-1031 (2008). ISSN: 1310-1331. IF – 0.204

20. Vangelov I. , Dineva J., Nikolov G, Gulenova D, Ivanova MRelationship among the anti- granulosa luteinized cells antibodies, the apoptosis of granulosa luteinized cells, the levels of TNF- α and IFN-γ in follicular fluids of infertile women. Comp. Rend. Bulg. Acad. Sci., 61: 341-348 (2008). ISSN: 1310-1331, IF-0.204

21. Mollova M, Martinova Y. Immunocytochemical localization of antigen involved in sperm-zona pellucida interaction. Acta morphologica et antropologica, 13: 98-101 (2008). ISSN: 0861-0509

22. Ruseva B, Tzvetkova R, Martinova Y, Mollova M, Alexandrova M, Lukanov T. Determination of glutathione peroxidase expression in aortic wall of spontaneously hypertensive rats under diet of different selenium content. J. Biomed. Clin. Res., 1 (1): 26-29 (2008).

23. Vaisberg Ch, Dishlianova B, Miteva L, Rashev P, Boychev M. Influence of exogenous applied active oxygen species on the reproductive indicators in male animals and possibilities for enhancement of fecundate ability of spermatozoa in artificial insemination of sheep. Bulgarian Journal of Agriculture Sciences, 14(5): 500-508 (2008). ISSN: 1310-0351

24. Vaisberg Ch, Jelezarsky L, Rashev P, Miteva L. Exogenous H2O2 increases motility of ram spermatozoa and the concentration of progesterone in blood serum of mice. Comp. Rend. Bulg. Acad. Sci., 61(8): 1021-1026 (2008). ISSN: 1310-1331, IF-0.204

25. Danailov K, Mollova M. Fusogenic activity ptoteins in gametes interaction. Reproductive Health, 15: 12-17 (2008). ISSN: 1312-6180

26. Terzieva V., Todorova Y, Drenska R., Markova R., Popova D, Abadjieva D., Elenkov I., Yankova M., Kostov K. Regulatory CD4+CD25+ Т cells, lymphocyte prolipheration and T cell response in НІV-1 infection. Modern Medecine, 3:25-35 (2008). ISSN 0562-7192

27. Danailov K, Rashev P, Zasheva D, Mollova M.. Immunochemical characterization of sperm-specific antigen with potential fusogenic activity in fertilization. Comp. Rend. Bulg. Acad. Sci., 62(2): 221-228 (2009). ISSN: 1310-1331, IF – 0.204

28. Martinova Y, Zaprjanova S, Rashev P, Mollova MExpression and localization of small heat shock proteins in mouse testicular tissue. J. Biomed. Clin. Res. Supp l1, 2: (1), 66-69 (2009).

29. Pupaki D., Sapundzhiev E, Rashev P, Stamenova M. Expression of low molecular weight stress-proteins (sHSPs) during tissue differentiation and organogenesis of pig fetuses. Acta Morphologica et Anthropologica, 15: (2009) под печат. ISSN: 0861-0509

30. Rashev P. Gelatinolytic activity and western blot analysis of matrix metalloproteinase (MMP)-2 and -9 in porcine uterine secretions during early pregnancy. Comp. Rend. Bulg. Acad. Sci., 62(5): 595-602 (2009). ISSN: 1310-1331 IF – 0.204

31. Rashev P. Localization of matrix metalloproteinases-2 and -9 in pig trophoblast and endometrium during early pregnancy. Comp. Rend. Bulg. Acad. Sci., 62 (8): 949-952 (2009). ISSN: 1310-1331,IF – 0.204

32. Vaisberg C.Rashev PGeorgiev BHristova V. Per os treatment with hydrogen peroxide as a method to increase testosterone level in rabbits. Comp. Rend. Bulg. Acad. Sci., 62 (12): 1535-1538 (2009). ISSN: 1310-1331, IF – 0.204

33. Zahariev P.J., Emil I. Sapundzhiev, Despina V. Pupaki, Pavel I. Rashev. Morphological characteristics of the stomach mucosa in carnivores. J. Biomed. Clin. Res. Suppl. 1, 2 (1): 66-68 (2009).

34. Vangelov I, Dineva J, Nikolov G, Gulenova D, Ivanova M. Impact of follicular cytokines (TNFα, IFNγ) and apoptosis of human granulosa luteinized cells (GLCs) on the results after controlled ovarian hyperstimulation (COH) and in vitro fertilization (IVF) in women with ovarian factor of infertility, Comp. Rend. Bulg. Acad. Sci., 62:1167-1174 (2009) ISSN: 1310-1331, IF-0.204

35. Borisov K., Savov A., Kremenski I., Raleva S. Markova R., Elenkov I., Terzieva V., Kostov K., Argirova R. Study of genetic polymorphism of the CCR5 chemokine receptor in HIV-infected Bulgarians. Infectology, 2: 32-34 (2009). ISSN 0861-8259

36. Georgiev P, Kinov P, Rashev P, Sapundzhiev E, Vidinov N. Changes in the distribution of fibrillar collagens during early healing of the lateral collateral ligament epiligament tissue in rat knee model. Comp. Rend. Bulg. Acad. Sci., 63 (5): 761-766 (2010). ISSN: 1310-1331, IF – 0.204

37. Dineva G, Vangelov I, Stefanova Tc, Gulenova D, Nikolov G, Ivanova M. Studies on the role of nitric oxide (NO) in pre-ovulatory human follicle. Reproductive Health (2010). (In print) ISSN: 1312-6180

38. Dineva G, Vangelov I, Abrashev R, Gulenova D, Nikolov G, Chaushev T Stamenov G, Angelova M, Ivanova M. Effective antioxidant enzyme protection of human granulose-lutein cells: a prerequisite for increased cell vitality and better results in infertile women included in program for IVF-ET. Reproductive Health (2010). (In print) ISSN: 1312-6180

39. Mollova M, Dimitrova D, Danailov K, Genov M, Stamenova M, Rashev P. Investigations on immunogenecity of the sperm-specific antigen AG60 involved in gamete fusion in infertile patients. Comp. Rend. Bulg. Acad. Sci., 64(1): (2011) ISSN: 1310-1331, IF – 0.204

40. Zaprjanova S, Rashev P, Zasheva D, Martinova Y, Mollova M. Electrophoretic analysis of HSP72 and HSP73 expression in male reproductive tissues. Comp. Rend. Bulg. Acad. Sci., 64(2): (2011). ISSN: 1310-1331, IF – 0.204

41. Dineva J, Vangelov I, Abrashev R, Gulenova D, NikolovG, Аngelova М, Ivanova М. Survival role of intracellular superoxide dismutases SODs and Catalase for human granulosa luteinized cells: A preliminary study. Comp. Rend. Bulg. Acad. Sci., 64(1): (2011) (in press) ISSN: 1310-1331, IF-0.204

 

Papers in Proceedings from International Scientific Meetings

1. Markova R., Drenska R., Terzieva V., Todorova Y., Stefanova D., Elenkov I., Yankova M. In vitro immunodiagnosis of MTB infection in HIV 1-/TB+ and TB+/HIV 1+ Bulgarian patients. ERS 15 annual congress, Copenhagen, Denmark, 2005, Abstr N P2901, публикуван в European Respiratory Journal, 26, suppl. 49, sept. 2005 IF – 3.947

2. Rashev. P., M. Mollova. Preliminary study on expression and localization of matrix metalloproteinase-2 (MMP-2) in ejaculated, capacitated and acrosome-reacted boar spermatozoa. Proceedings IV Balkan Conference of Animal Science BALNIMALCON 2009. Challenges of the Balkan Animal Industry and the Role of Science and Cooperation, pp. 189-191, 2009

3. Dineva J., Vangelov I., Terzieva V., Gulenova D., Nikolov G., Ivanova M.D. The immunosuppressive role of the atrial natriuretic peptide (ANP) in preovulatory human follicle; 8th European Congress on reproductive immunology, abstr. # 8, 11-13 nov. 2010, Munich, Germany, published in J. Reprod. Immunol. November 2010 (Vol. 86, Issue 2, Page 83). IF – 2.159 (2009)

Papers in Proceedings from Bulgarian National Scientific Meetings

1. Nikolov I, Sabev M, Kicheva M, Rashev P, Gerzilov C, Hristev Hr. Studies on the protective role of low molecular weight lipoprotein fraction (LDL) on the cryotolerance of ram sperm.Scientific Works of the AU-Plovdiv, Volume LII: 79-86 (2006).

2. Sabev M, Nikolov I, Ivanova-Kicheva M, Petrov M, Rashev P. Influence of sugars as components in diluents on the viability of boar sperm under in vitro storage conditions.In: Reports Collection from the Conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 197-201 (2006).ISSN: 1313-4337.

3. Zelezarski L, Rashev P, Sapoundjiev F, Weissberg Ch. Localization of GGT in internal genital organs of boars.In: Proceedings of the conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 237-242 (2007).ISSN: 1313-4337.

4. Zelezarski L, Weissberg Ch, Rashev P. GGT activity in sperm from boars and production of an antibody against GGT. In: Proceedings of the conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 230-236 (2007).ISSN: 1313-4337.

5. Nikolov I, Sabev M, Stefanov P, Ivanova-Kicheva M, Hristov H., Rashev P. Protective role of a LDL-fraction of egg yolk in different technologies for cryopreservation of sperm of the genus Ovis Aries.In: Proceedings of the conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 218-223 (2007).ISSN: 1313-4337.

6. Pupaki D, Sapoundjiev E, Rashev P, Stamenova M. Expression of a-crystallin in histogenesis of pig fetuses.In: Proceedings of the conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 30-36 (2007).ISSN: 1313-4337.

7. Sabev M, Stefanov P, Angelova M, Nikolov And Kazachka E, Ivanova-Kicheva M Rashev P. protective role of antioxidants on sperm of the genus Sus Scrofa in terms of in vitro storage.In: Proceedings of the conference “Traditions and Modernity in veterinary medicine”, UF, FVM, 224-229 (2007).ISSN: 1313-4337.

8. Hristo Hristov, Nikolov And Sabev M, Ivanova-Kicheva M, Stefanov P Rashev P. Influence of environmental factors on sperm from kriorezistentnostta eyakulati ram in izvanrazmnozhitelniya season. “Ecological problems of Bulgarian agriculture in the European Union” Agroeko 2007, AU – Plovdiv, Volume LII, 309-314 (2007).

9. Rashev P Zahariev P Pupaki E Sapoundjiev F. immunohistochemical typing of collagen composition of the stratum compactum of the lining of the feline stomach. Proceedings of the conference “Traditions and Modernity in veterinary medicine, UF, FVM, 28-33 (2008).ISSN: 1313-4337.

10. Rashev P, Mollova M. Preliminary study on expression and localization of matrix metalloproteinase-2 (MMP-2) in ejaculated, capacitated and acrosome-reacted boar spermatozoa.Proceedings IV Balkan Conference of Animal Science BALNIMALCON 2009. Challenges of the Balkan Animal Industry and the Role of Science and Cooperation, 189-191 (2009).

11. Dineva G Vangelov And Gregoraschuk E Augustowski K, Ivanova M. Studies on the gonadotropin-mediated expression of atrialen natriuretic peptide, progesterone production and apoptosis in porcine granulosa cells, In: Proceedings at the University of Forestry (2009) (in press) ISBN 954-332-014-4

 

Popular Science, Current Affairs publications and Educational publications 

  I. Books and brochures

1. Ivan Nikolov and Pavel Rashev. Guidance for biology of reproduction and artificial insemination in farm animals. Academic edition of Agricultural University – Plovdiv, Plovdiv, 168 pages, 35 figures, 6 tables, 2008 (in Bulgarian)ISBN 978-954-046-1.

2. 70th Anniversary Institute of Biology and Immunology of Reproduction “Acad. K. Bratanov” 1938-2008, Sofia, Bulgaria, 75 pages.

3. Lolov S. Othosclerosis (monography), Ciela, Sofia, 304 pp, 69 figs, 17 tabls., 4 suppls, (2010). ISBN: 9789542808787

 

II. Artilces

 1. Mollova M. Second national round table on problems of reproductive biology in farm animals in Bulgaria. Newsletter of the Academy of Sciences, No. 10, p. 80-10 (2007). ISSN: 1312-5311

2. Mollova M. Scientific achievements of the Institute of Biology and Immunology of Reproduction in the field of reproductive biology. Animal breeding Magazine, № 4, 6-8 (2007). ISSN: 0204-7225

3. Mollova M. Georgieva R. IBIR – Leading Research Center in Biology and Immunology of Reproduction problems. BM news magazine, kn.11-12, 42-43 (2008), ISSN 1311-0284

4. Mollova M, Georgieva R. Institute of Biology and Immunology of Reproduction. Newsletter of the Academy of Sciences, No.1, 13-15 (2009). ISSN: 1312-5311

5. Georgieva P, Mollova M. 12th International Symposium on Immunology of Reproduction, 22 to 27 June 2009. Newsletter of the Academy of Sciences, Vol. 9, 5-8 (2009). ISSN: 1312-5311

6. Mollova M, Konova E, Kyurkchiev St. Reproduction Research Center. Advances in Bulgarian Science, Body of the National Centre for Information and Documentation at the Ministry of Education. 28-31 (2009). ISSN 1312-6164

7. Mollova M. Immunology of reproduction-an interview with “Forum Medicus” newspaper, a body of medical societies in Bulgaria. No.33-34, 14.09.2009., Year LXIV, p. 10 (2009).

8. Mollova M. Academician Kiril Bratanov – still alive in the minds of his students and followers, the newspaper Homo Sciences, Section: Bulgarian scientific schools, published UBS, No.3, p. 6 (2009). ISSN 13128884

9. Mollova M R. Georgieva “The Bulgarian Trace in Science” Section: Biological Sciences. Poster exhibition brochure for the work of Academician K. Bratanov Initiative included in the Project Star Night, funded by 7 FP of the EU Commission: “Researches Night 2009”.

 

Participation in Scientific Meetings

1.1. International Scientific Meetings

1. Terzieva V., Martinova F, Markova R., Todorova Y., Drenska R., Elenkov I., Yankova M., Kostov K. Influence of CD4+CD25+ regulatory T cells on HIV-1 specific CD4+ T cell responses in HIV-1 infected Bulgarian patients. 10th European AIDS conference/EACS, November 17-20, Dublin, Ireland, Abstr N PE 2.4/1. (2005)

2. Markova R., Drenska R., Terzieva V., Todorova Y., Elenkov I., Yankova M., Kostov K., Stefanova D. Evaluation of ESAT-6 & CFP-10 – specific T lymphocytes in co-infected with HIV-1 and M. tuberculosis Bulgarian patients during specific anti-tuberculosis therapy. 3rd International AIDS Society Conference on HIV Pathogenesis and Treatment. Rio de Janeiro, July 24-27. Abstract No TuPe7.1C02. (2005)

3. Markova R., Drenska R., Terzieva V., Todorova Y., Stefanova D., Elenkov I., Yankova M. In vitro immunodiagnosis of MTB infection in HIV 1-/TB+ and TB+/HIV 1+ Bulgarian patients. ERS 15 annual congress, Copenhagen, Denmark, Abstr N P2901 (2005)

4. Dineva J, Wojtowicz A, Augostowska K, Vangelov I, Gregoraszuk E,. Ivanova M. Expression of Atrial Natriuretic Peptide (ANP), Progesterone (P), Bcl-2 and p53 in in vitro Luteinized and Leptin treated porcine granulose cells in relation with apoptosis, Graz, 5-9 July, AJRI, 56: 34-61 (2006).

5. Gartner W, Vila G, Daneva T, Koc-Saral F, Majdic O, Nabokih A, Apostolova M, Luger A, Wagner L. Characterization and detectability of the rat homologue of the human neuroendocrine marker protein secretagogin. 9th Meeting of the European Calcium Society, Strasbourg, 19 – 22. VII. (2006).

6. Stamenova M, Martinova Y, Zapryanova S, Rashev P, Mollova M. Expression of sHSP in male germ cells during mouse spermatogenesis. 3rd Eur. Congr. Immunol. Reprod. Dev., Graz, 5-9 July, AJRI, 56 (7): 34-61 (2006).

7. Vangelov V, Dineva J, Nikolov G, Genov M, Ivanova M. The relationship of Anti-granulosa cell antibodies (AGA) in follicular fluids (FFls) of women with the IVF outcome, Graz, 5-9 July, AJRI, 56: 34-61 (2006).

8. Terzieva V., Todorova Y., Drenska R., Markova R., Martinova F., Elenkov I., Yankova M. Influence of CD4+CD25+ regulatory T cells on the degree of immune activation in HIV-1-infected Bulgarian patients; 16th World AIDS Conference, Toronto, Canada, 13-18 August . Abstr. No CDA0086. (2006)

9. Todorova Y., Markova R., Drenska R., Terzieva V., Elenkov I., Yankova M. Evaluation of cell-mediated immune function in therapy free HIV-1/AIDS Bulgarian patients. 1st Joint Meeting of European National Societies of Immunology and 16th European Congress of Immunology Sept. 2 – 6, Paris, France, PC-1537 (2006)

10. Drenska R., Markova R., Todorova Y., Terzieva V., Stefanova D. PPD – stimulated cytokine production in patients with M. tuberculosis infection, Absrt. No PA-3292; 1st Joint Meeting of European National Societies of Immunology and 16th European Congress of Immunology Sept. 2 – 6, Paris, France (2006)

11. Markova R., Drenska R., Todorova Y., Terzieva V., Stefanova D., Elenkov I., Yankova M. Utility of antigen-specific IFN-γ assays for monitoring of disease activity and efficacy of anti-TB treatment in HIV-/TB+ and HIV+/TB+ patients in Bulgaria. 16th Congress of ERS, Munich, 02.09 – 06.09., Abstr. No E 2042. (2006)

12. Markova R., Todorova Y., Drenska R., Terzieva V., Elenkov I., Yankova M. Use of two commercial interferon-gamma assays for detection of tuberculosis infection among HIV/AIDS patients in Bulgaria. 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention, Abstract no. MOPEA094. (2006)

13. Markova R., Drenska R., Todorova Y., Terzieva V., Stefanova D. Monitoring the efficacy of anti-TB therapy by using the QuantiFERON-TB Gold in tube test. 16th Congress of ERS, Munich, 02.09 – 06.09., Abstr. No E1689. (2006)

14. Dineva J, Vangelov I, Nikolov G, Konakchieva R, Stefanova Ts, Ivanova M. Study on the survival role of ANP-cGMP-P and NO in human periovulatory follicle, X International Congress of Reproductive Immunology, Opatija, Croatia, June 10-14, (2007).

15. Dineva J, Vangelov I, Konakchieva R, Stefanova T, Ivanova M, Studies on the immunoendocrine regulation of cell survival and apoptosis in preovulatory human follicle, V European congress of Reproductive immunology, Berlin, 3-7 September, (2007).

16. Dineva J, Vangelov I, Konakchieva R, Stefanova Ts, Ivanova M. Studies on the immunoendocrine regulation of cell survival and apoptosis in preovulatory human follicle, XIII Symposium of Czech Reproductive Immunologists with international participation, Zdar, Chech Republic, 24-27 May (2007).

17. Gartner W, Vila G, Daneva T, Koc-Saral F., Majdic O, Nabokih A, Luger A, Wagner L. Characterization of the rat homologue of the human neuroendocrine marker secretagogin – new functional implications by in vitro studies. 9th European Congress of Endocrinology, Budapest, Hungary, 28. IV – 02. V. (2007).

18. Gartner W, Vila G, Daneva T, Koc-Saral F., Majdic O, Nabokih A, Luger A, Wagner L. Characterization of the rat homologue of the human neuroendocrine marker secretagogin – new functional implications by in vitro studies 51st Annual Meeting of the German Society of Endocrinology (DGE), Salzburg, Austria 7-10. III. (2007).

19. Mollova M., Stamenova M., Rashev P. Biochemical nature of boar sperm antigen with functional activity in primary sperm-zona pellucida binding. X International congress of reproductive immunology, Opatija, Croatia, June 10-14 (2007).

20. Mollova M., Zaprjanova S, Rashev P, Martinova Y. Developmental and hyperthermia-related expression of heat shock protein 70 (HSP70) in mouse testis. XIII Symposium of Czech Reproductive Immunologists with International Contribution, Zdar nad Sazavou. May 24-28 (2007).

21. Drenska R., Markova R., Todorova Y., Terzieva V., Stefanova D. ESAT-6/CFP-10 and PPD-stimulated cytokine production in patients with MTB infection. 16th Congress of ERS, Munich, 02.09 – 06.09., Abstr. No 4447. (2007)

22. Terzieva V., Todorova Y., Popova D., Drenska R., Markova R., Martinova R., Elenkov I., Yankova M.: Correlation entre la production d’IL-2 par des cellules T CD4+ et la fonction VIH-specifique des cellules T regulatrices; 4e Conference francophone VIH/SIDA, 29-31 Mars, Abstr. No 360/57p. (2007)

23. Todorova Y., Markova R., Drenska R., Terzieva V., Elenkov I., Yankova M. Evaluation of T-cell function in HIV-1 Bulgarian patients under HAART. 8th John Humphrey Advanced Summer Programme in Immunology, September, Moscow, Russia. (2007)

24. Terzieva V., Todorova Y., Markova R., Drenska R., Popova D., Abadjieva D., Martinova F., Elenkov I., Kostov K., Yankova M.: IL-2 Strongly influences the ability of Tregs to reduce immune activation and to specifically produce IL-10 in HIV-1 infected subjects under HAART: 11th European AIDS Conference/EACS, October 24 – 27, , Madrid, Spain, Abstr. No P6.9/02 (2007)

25. Terzieva V., Popova D., Kitcheva M., Todorova Y., Markova R., Martinova F., Elenkov I., Yankova M. Correlation between the degree of immune activation, production of IL-2 and FOXP3 mRNA levels in CD4+CD25+ Treg cells in HIV-1 infected subjects under HAART. T regs Conference, China, PO O170. (2008)

26. Seghrouchni F, Contini S, Markova R, Drenska R, Sadki K., Baassi L., Todorova Y, Terzieva V, Bocchino ML, Altieri A., Cappelli G, Alma M G, Mariani F, Benjouad A., Colizzi V, Aouad R E, Saltini C, Amicosante M. Design of immunogenic multiepitopic Mycobacterium tuberculosis peptides from genes selected using in vitro models of mycobacterial macrophage infection. 1st Interantional Conference of Immunodiagnosis of Tuberculosis, Sept. 21-23, USA, Abstract book, p. 36. (2008)

27. Ivanova M, Dineva J, Vangelov I, Konakchieva R, Terzieva V. Studies on the local immunoendocrine regulation of cell survival in preovulatory human follicle. 7th European Congress on Reproductive Immunology. Marathon, Greece, September 17-20 (2009).

28. Dineva J., Vangelov I., Terzieva V., Gulenova D., Nikolov G., Ivanova M.D. The immunosuppressive role of the atrial natriuretic peptide (ANP) in preovulatory human follicle; 8th European Congress on reproductive immunology, abstr. # 8, 11-13 nov. 2010, Munich, Germany, published in J. Reprod. Immunol. November 2010 (Vol. 86, Issue 2, Page 83), IF- 2.159 (2009)

29. Dineva J, Vangelov I, Terzieva V, Nikolov G, M. Ivanova M. The immunosuppressive role of the Atrial Natriuretic Peptide (ANP) in preovulatory human follicle. 8th European Congress of Reproductive Immunology, Munich, Germany, November 11-14 (2010). JRI, 86(2): ISSN 0165-0378, IF – 2.536

30. Mollova M. Sperm-specific antigen with fusogenic activity and its role in immunological infertility. Meeting at University Clinic for Endocrinology and Reproduction, Germany, Magdeburg, 22.09 – 29.09.(2010).

31. Mollova M., M.Stamenova, S.Zaprjanova, Y.Martinova, P.Rashev, R.Dimitrov. Develpmental and cell type – specific expression of α,B crystalline (α,B-cry) in mouse testis and epididymis. 8th EuropeanCongress on Reproductive ImmunologyMunich, Germany, November, 11-14 (2010). JRI, 86 (2): ISSN 0165-0378, IF – 2.536

32. Mollova M., M.Stamenova, S.Zaprjanova, Y.Martinova, P.Rashev, R.Dimitrov. Develpmental and cell type-specific expression of α,B crystalline (α,B-cry) in mouse testis and epididymis 8th European Congressof Reproductive Immunology (ESRADI), Munich, Germany, November 11-13 (2010), Abstr.31, JRI, 86 (2): ISSN 0165-0378, IF – 2.536

33. Sainova R, Nitsh A, Nitsh I, Vavrek V, Pavlova S, Daneva T, Manchev S, Nikolova E. Experimental model for safe applications of laboratory-cultivated stem and progenitor cells, transfected by recombinant gene constructs. RNA Quality Control EMBO Workshop, Vienna, Austria, 06. 2010 – 13. 06. (2010).

34. Vangelov I, Dineva J, Todorova K, Stefanova Ts, Nokolov G, Gulenova D, Ivanova M. Relationship of follicular fluid oxide concentrations with the serum steroid (progesterone, estradiol) levels, apoptosis of granulose luteinized cells and with the outcomes after COH/IVF. 8th European Congress on Reproductive Immunology, Munich, Germany,November 11-13 (2010).JRI, 86 (2): ISSN 0165-0378, IF – 2.536

35. Zahariev P, Pupaki D, Sapundzhiev E, Rashev P, Palov A, Todorov T. Morphologic characteristic of the layer stratum compactum in feline stomach mucosa. XXVIIIth EAVA Congress – Paris, France, P2., 628, (2010).

36. Terzieva V., Todorova Y., Popova D., Vladimirova R., Kitcheva M., Elenkov I., Yankova M. Regulatory T cells control immune activation and HIV-1 specific IFN-g responses and depend on IL-2 production from conventional T cells;, Abstr. No THPE0050, 18th International AIDS Conference, Vienna, 18-23 July (2010).

 

1.2. Bulgarian National Scientific Meetings

 

  1. Vangelov I, Dineva J, Nikolov G, Ivanova M. Relationship of anti-granulosa antibodies (AGA) in follicular fluids (FFs) of infertile women with the response of controlled ovarian hyperstimulation (COHS) and the level of fertilization after IVF-ET. 6th National Congress of Infertility, Contraception and Hormone replacement Therapy, Borovetz, 13-16 March 2005
  2. Dineva J, Vangelov I., Konakchieva R., Nikolov G, M. Ivanova. Apoptosis of granulosa luteinized cells (GLCs) and cumulus cells (CCs) associated with the age of women in the IVF program “. 6th National Congress of Infertility, Contraception and Hormone Replacement Therapy, Borovetz, 13-16 March 2005
  3. Dineva J, Vangelov I, Nikolov G, Konakchieva R, Martinova Y, Ivanova M, In vitro study on the role of progesteron, cGMP and EGF on granulosa cells survival. 11th Intern. Symp. Immunol.of Reprod., Varna, 2-4 Sept. 2006
  4. Ivanova М, Dineva J, Vangelov I. Intraovarian regulators of granulosa cells suvival and apoptosis. 11th International Symposium of Immunology of Reproduction. Varna, June 2-4, 2006
  5. Koc F, Gartner W, Birkenkamp-Demtroeder K, Altenberger T, Mineva I, Daneva T, Wagner L. Identification of a novel RUVBL1/TIP49a variants with potential implications on chromosomal segregation. Varna, 11th International Symposium for Immunology of Reproduction, 2- 4. VI. 2006
  6. Mollova M, Stamenova M. Molecular basis of sperm-egg adhesion/fusion. 11th International Symposium of Immunology of Reproduction, Varna, 2-4 June 2006
  7. Mollova M, Martinova Y. Immunocytochemical localization of antigen involved in sperm-zona pellucida interaction. In: Sixth International Conference of Morphology and Antropology , Koprivshtiza, 2-June, 2006
  8. Vangelov I, Dineva J, Nikolov G, Ivanova M, Аnti-granulosa antibodyes (AGA) in follicular fluids (FFls) and their relationship with response to COH and fertilization rate, Varna, 11th. XI international Symposium of Immunology of Reproduction, Varna, 2-4 June 2006
  9. Zapriyanova S, Rashev P, Martinova Y, Mollova M. Expression and localization of Hsp70 in mouse germ cells. XI international Symposium of Immunology of Reproduction, Varna, 2-4 June 2006.
  10. Dineva J, Vangelov I, Nikolov G, Gulenova D, Ivanova M. Levels of apoptosis of granulosa luteinized cells (GLCs) and cumulus cells (CCs) as a possible indicator of outcome in women undergoing assisted reproduction. 7th National Congress of Infertility, Contraception and Hormone Replacement Therapy, Borovetz, 19-23 March 2006
  11. Nikolov I, Sabev M, Kicheva M, Rashev P, Gerzilov V, Hristev Hr. Studies on the protective role of low molecular weight lipoprotein fraction (LDL) cryotolerance of ram sperm. Scientific Conference of the Agricultural University in Plovdiv, Plovdiv, Bulgaria, 15-16.12. 2006
  12. Sabev M, Nikolov I, Ivanova-Kicheva M, Petrov M, Rashev P. Effect of sugars on the viability of boar sperm in in vitro storage. Scientific Conference, UF, Sofia, November 24, 2006.
  13. Trifonova N, Angelov B, Popovska C, Beethoven T, Rashev P, Sapoundjiev E, Stamenova M. Expression of antigens in ocular lens beyond eye tissues of human embryo. XXV Jubilee Annual Conference of the Sofia branch of the Bulgarian Society of Ophthalmology, Sofia, 24-25 November 2006.
  14. Martinova Y, Mollova M, Petrov M, Rashev P, Ivanova M. TEM and SEM characteristics and lectin binding distribution of cat zona pellucida during oocyte maturation and fertilization. ХVІІІ National Congress of the Bulgarian Society of Anatomy, Trakia Journal of Sciences, 5 (1) Suppl. 2, Stara Zagora, 1-3 June, 2007.
  15. Martinova Y, Rashev P, Zaprjanova S, Mollova M. Early ultrastructural changes in mouse testis and epididymis after single exposure to heat. International Congress of Andrology “Prostate-the men’s Achilles heel”, Varna, May17-19, 2007
  16. Mollova M., Stamenova M, Rashev P, Ivanova M. Anti-sperm antibody with anti- fertile activity in human gamete interaction in vitro. 8th National Congress on sterility, Contraception, Hormone Replacement Therapy and Gynaecological Endoscopy with International Participation, Borovetz, Hotel “Samokov”, 17-19 March, 2007.
  17. Genov M, Vangelov I, Dineva J, Ivanova M. Anti ovarian antibodies and approaches to overcoming immunologically related infertility. 8th National Congress of Infertility, Contraception and Hormone Replacement Therapy. Borovetz 17-19 March 2007
  18. Dineva J, Vangelov I, Konakchieva R, Nikolov G, Ivanova M. Comparative studies on the indexes of success in patients treated with GnRHa (Decapeptil) and GnRHant (Oragalutran) in IVF program
  19. Zhelezarski L, Rashev P, Sapoundjiev E, Weissberg Ch. Localization of GGT in internal genital organs of boars. Scientific Conference of the VMF at UF, Sofia, November 23, 2007
  20. Zhelezarski L, Weissberg Ch, Rashev P. GGT activity in boar semen. The obtaining of antibody against GGT. Scientific Conference of the VMF at UF, Sofia, November 23, 2007
  21. Martinova Y, Rashev P, Zapryanova S, Mollova M. Ultrastructure changes in the testis and epididymis of mice after single heat effects. International Congress of Andrology “The Prostate – Achilles heel of man and IIIth National Congress of Andrology, Golden sands, Varna, Bulgaria, 17-19 May 2007.
  22.  Mollova M. Scientific achievements of the Institute of Biology and Immunology of Reproduction in the field of reproductive biology. Second national round table on problems of reproductive biology in farm animals in Bulgaria, the Great Hall of the Academy of Sciences, June 20, 2007.
  23. Nikolov I, Sabev M, Stefanov R, Ivanova-Kicheva M, Hristov H, Rashev P. Protective role of LDL-fraction of egg yolk in different technologies for cryopreservation of sperm of the genus Ovis Aries. Scientific Conference of the VMF at UF, Sofia, November 23, 2007
  24. Pupaki D, Sapoundjiev E, Rashev P, Stamenova M. Expression of -a crystallin in histogenesis of swine fetuses. Scientific Conference of the VMF at UF, Sofia, November 23, 2007
  25. Sabev M, Stefanov R, Angelova M, Nikolov I, Katsachka E, Ivanova-Kichev M Rashev P. Protective role of antioxidants on sperm of the genus Sus Scrofa in terms of in vitro storage. Scientific Conference of the VMF at UF, Sofia, November 23, 2007
  26. Hristov Hr, Nikolov I, Sabev M, Ivanova-Kicheva M, Stefanov P, Rashev P. Influence of environmental factors on cryo resistance of ram sperm in out breeding season. Eighth scientific and practical conference with international participation “Ecological problems of Bulgarian agriculture in the European Union” Agroeko 2007, AU – Plovdiv, 17-18 May 2007.
  27. Danailov K, Rashev P. Localization and tissue specificity of sperm protein with fusogenic activity in fertilization. IIIrd Koprivshtitsa Morphological Days and VIIth National Conference of Anthropology May 30 – June 1, 2008.
  28. Danailov K, Rashev P, Zasheva D, Mollova M. Characterization of sperm-membrane antigen with ponentioal fusogenic activity in fertilization. Scientific session dedicated to the 70th anniversary of the Institute of biology and immunology of reproduction “Acad. K. Bratanov”, with International Participation, 22-23 October, 2008.
  29. Dineva J, Vangelov I, Terzieva V, Nikolov G, Ivanova M. Study on the immunomodulatory and antiapoptotic role of Atrial natriuretic peptide (ANP) in human ovary. София, 23 Октомври 2008
  30. Mollova M. Achievements and perspectives for development of Institute of biology and immunology of reproduction “Acad. K. Bratanov”. Scientific session dedicated to the 70th anniversary of the Institute of biology and immunology of reproduction “Acad. K. Bratanov”, with International Participation, 22-23 October, Sofia , 2008
  31. Pupaki D., Sapundzhiev E, Rashev P, Stamenova M. Expression of low molecular weight stress-proteins (sHSPs) during tissue differentiation and organogenesis of pig fetuses. IIIrd Koprivshtitsa Morphological Days and VIIth National Conference of Anthropology May 30 – June 1, 2008.
  32. Zaprianova S, Rashev P, Mollova M, Martinova Y. Immunodetectiom of HSP70 during differentiation and maturation of mouse male germ cells. Scientific session dedicated to the 70th anniversary of the Institute of biology and immunology of reproduction “Acad. K. Bratanov”, with International Participation, 22-23 October, Sofia , 2008
  33. Zaprjanova S, Rashev P, Martinova Y, Mollova M. Expression and immunocytochemical localization of heat shock protein 70 (HSP 70) during different stages of mouse sperm maturation. IIIrd Koprivshtitsa Morphological Days and VIIth National Conference of Anthropology, with International Participation, May 30 – June 1, 2008.
  34. Vangelov I, Dineva J, Nikolov G, Gulenova D, Ivanova M. Relationship of follicular granulosa levels of anti-antibodies (AGA), TNFα, IFNγ and apoptosis of granulosa luteinized cells (GLCs) with the results of KOH / IVF in infertile women. IX National Congress of Infertility, Contraception and Hormone Replacement Therapy, VK Riviera, 11-14 April 2008
  35. Danailov K, Rashev P, Zasheva D, Mollova M. Characterization of membrane-associated sperm antigen with potential fusogen activity in fertilization. Scientific session dedicated to 70 years of the Institute of Biology and Immunology of Reproduction “Acad K. Bratanov. October 22 to 23, Sofia, 2008
  36. Seghrouchni F, Contini S, Markova R, Drenska R, Todorova Y, Terzieva V, Cappelli G, Alma M G, Mariani F, Petrunov B, Colizzi V, Aouad R E, Saltini C, Amicosante M. Design of immunogenic multiepitopic Mycobacterium tuberculosis peptides from genes selected using in vitro models of mycobacterial macrophage infection, 29th Annual Congress of the European Society of Microbiology, July 6–9, 2008, Plovdiv, Bulgaria Abstract No OP-34
  37. Rashev P, Zahariev P, Pupaki D, Sapoundjiev F. Immunohistochemical typing of collagen composition of the stratum compactum of the of the feline stomach mucosa. Scientific Conference of the Navy at UF, Sofia, November 21, 2008
  38. Ruseva B, Ivanova R, Martinova Y, Mollova M, Lukanov Ts. Effect of the different selenium diet on the glutathione-peroxidase expression in the aorta of the norm-and-spontaneously hyperactive rats. IV National Conference on Nutrition “Novel foods, nutritional supplements and drugs in the prevention and treatment of modern diseases”, Sofia, 30-31 October 2008.
  39. Danailov K, Ivanova M, Petrov M, Zasheva D, Mollova M. Biological characterization of sperm 60 kDa antigen. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, 25-27 June 2009.
  40. Dimitrova D, Danailov K, Genov M, Stamenova M, Mollova M. Immunogenicity of the sperm-specific antigen involved in gamete fusion in infertile patients. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, 25-27 June 2009.
  41. Dineva J, Vangelov I, Nikolov G, Gulenova D, R. Konakchieva Stefanova Tz, Ivanova M. The atrial natriuretic peptide- cGMP- progesterone and nitric oxide as a pro- survival factors for human granulosa luteinized cells. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, June 25- 27, 2009.
  42. Georgiev B., Rashev P, Shumkov K, Taushanova P, Kacheva D. Morphological characterization of boar semen in long-time storage at 15°C. 12th International Symposium of Immunology of Reproduction, PII-18, Varna, Bulgaria, 25-27 June 2009.
  43. Ivanova M, Dineva J, Vangelov I, Konakchieva R, Terzieva V. Studies on the local immunoendocrine regulation of cell survival in preovulatory human follicle 12th International Symposium of Immunology of Reproduction, Varna, June 25- 27, 2009.
  44. Martinova Y, Zaprianova S, Mollova M, Rashev P, Tzvetkov D. Mechanisms of male germ cell apoptosis in human infertile cryptorchid testis. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, 25-27 June 2009.
  45. Mollova M, Stamenova M. Role of HSP’s in mechanisms of spermatogenesis and apoptosis (plenary lecture). 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, 25-27 June 2009.
  46. Mollova M., Danailov K,.Dimitrova, Stamenova M, Genov M, Rashev P. The role of sperm-specific antigens with fusogenic activity in gamete interaction for immunologically-dependent human infertility. X Anniversary Congress of Infertility, Contraception, Hormone Replacement Therapy and Ginecological Endoscopy, with International Participation, 23-26 April, Nessebar, 2009.
  47. Rashev P, Mollova М. Preliminary study on expression and localization of matrix metalloproteinase-2 (MMP-2) in ejaculated, capacitated and acrosome-reacted boar spermatozoa. Challenges of the Balkan Animal Industry and the Role of Science and Cooperation, Trakia University, Faculty of Agriculture, Stara Zagora, Bulgaria, IV Balkan Conference of Animal Science Banimalcon 2009.
  48. Rashev P. Gelatinolytic activity and western blot analysis of matrix metalloproteinase (MMP)-2 and -9 in porcine uterine secretions during early pregnancy. 1st Conference of the Balkan Network for the Animal Reproduction Biotechnology. p. 44, Sofia, Bulgaria, February 18-19, 2009.
  49. Ruseva B, Ivanova R, Dimitrova A, Laleva P, Martinova Y, Mollova M. Changes of glutathione peroxidase expression in aortic wall of normotensive and hypertensive rats under diet of low and high selenium content. XIX National Congress of Bulgarian Anatomical Society with international participation, Pleven, 29-31 May, 2009.
  50. Vangelov I, Dineva J, Nikolov G, Gulenova D, Genov M, Ivanova M. Impact of TNF-α and IFN-γ, and apoptosis of human granulosa luteinized cells on the results after ovarian stimulation/in vitro fertilization in women with ovarian factor of infertility. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, June 25- 27, 2009
  51. Zahariev P., Sapundzhiev E, Pupaki D, Rashev P. Morphologic characteristics of stomach Mucosa in carnivores. XIX National Congress of Bulgarian Anatomical Society with International Participation, Pleven, 29 – 31 May, 2009.
  52. Zaprianova S, Rashev P, Martinova Y, Mollova M. Effect of hyperthermia on mouse spermatogenesis and apoptosis. 12th International Symposium of Immunology of Reproduction, Varna, Bulgaria, 25-27 June 2009.
  53. Zaprianova S, Rashev P, Mollova M., Martinova Y. Expression, localization and homology of heat shock protein 70 in human and mouse testis. XI Anniv.Scient.Conference with International Attendance “Biology – Traditions and Challenges”, May 27-29, 2009
  54. Zaprjanova S, Rashev P, Mollova M, Stamenova M, Martinova Y. Expression and localization of small heat shock proteins in mouse testicular tissue. XIX National Congress of Bulgarian Anatomical Society with International Participation, Pleven, 29 – 31 May, 2009
  55. Dimitrova D, Danailov K, Rashev P, Genov M, Stamenova M, Mollova M. Importance of etiopathogenesis of immunological determined infertility in human sperm-specific antigen involved in the gametes fusion. Eighth National Conference on Medical Biology., Sofia, 2-4 October 2009
  56. Mollova M, Danailov K, Dimitrova D, Stamenova M, Genov M, Rashev P. Role of the sperm-specific antigens related to gametes fusion in the etiopathogenesis of immunological determined human infertility. 10th National Congress of infertility, contraception, hormone replacement therapy and gynecological endoscopy with international participation, OC-08, Sol Nessebar Resort, 23-26 April 2009.
  57. Ivanova M.D., Dineva J., Vangelov I., Konakchieva R., V. Terzieva. Studies on the local immunoendocrine regulation of cell survival in human preovulatory follicle. 12th International Symposium for Immunology of Reproduction, 25 – 27 June 2009, Varna, Abstract book.
  58. Georgiev G, Vidinov N, Rashev P, Sapundzhiev E, Kinov P. Immunohistochemical Study for Type I, III and V Collagens During the Early Healing of the Isolated Injury of the Lateral Collateral Ligament Epiligament Tissue in Rat Knee Model. XI National Congress of Orthopedics and Traumatology with international participation, M