Embryobitechnologies in animals

  • Main research topics

    Head of Department:  Associate Professor ROSSEN GEORGIEV STEFANOV, DVM, PhD

    Main research topics

    • Research on reproduction in farm animals under naturally occurring conditions and elaboration of novel methods and approaches to facilitate maximum use of their reproductive capacity.
    • Investigations on processes of superovulation and synchronization of the oestrous cycle between donors and recipients (cattle, sheep, goats, buffaloes), induced by different hormonal preparations and schedules during oestral, early and late anoestral season, with the aim to improve the embryobiotechnology in different animal species.
    • Elaboration of surgical (laparotomy, laparoscopy) and non-invasive methods for the receipt and transfer of embryos (in mice, rabbits, sheep, goats and buffaloes).
    • Morphological examinations of the special features of oocytes and embryos of different animals at cellular and sub-cellular level, as well as species idiosyncrasy in the dynamic of the pre-implantation embryo development.
    • Development of methods for fresh embryo culturing invitro and invivo and cryopreservation methods for long-term storage.
    • Investigations on the impact of biologically active substances of non-hormone origin on the reproductive potential of farm animals.
    • Scientific achievements

      Most significant scientific achievements during last 10 years

      • Successful inter-breed transfer of embryos of endangered aborigine Bulgarian animal breeds has been carried out. In aid of preserving the national genefund, an experimental cryobank of cryopreserved embryos of aborigine national breeds of sheep, goats and cattle has been established.
      • By observing the toxic dynamic and biochemistry of several xenobiotics (nitrates, phosphoorganic substances and synthetic pyrethroids) and their influence on the glutathione-dependent defense system operating in reproductive organs, it’s been found that the system is linked to detoxification of the endogenous and exogenous xenobiotics and has great importance in processes of xenobiotics’ biotransformation and cell protection against their toxic effects
      • Studies aimed at clarification of the toxic biology of reproductive processes revealed decreased activity of the enzymes from the glutathione family (GST, TSH, NP-SH, GSH) in the uterus and ovaries of experimental animals. Reduced activity of the enzymes may result in corresponding attenuation of defense cellular functions against xenobiotics influence.
      • Laboratory model of lung neoplastic disease in sheep has been introduced (lung adenomathosis) which has been proved as a zoonosis. The model has found application by sheep immune prophilaxis and by examinations of other similar viral infections in ruminants and adenocarcinoma in men, which is manifested in association with certain professional occupation in unfavourable milieu.
      • Methodology has been introduced for the establishment of safety control and prevention of risks for inoculation with viral pathogens from germinal plasma (oocytes, spermatozoa, embryos) by manipulations during the conduction of reproductive biotechnologies. Application of embryotransplantation has been adopted as a strategy for the achievement of healthy generation from ill donors of valuable breeds.
      • Influence of ultra-low temperatures on the ultra-structural organization of intracellular Ca++ in ram spermatozoa has been examined. Differences in the quality, amount and distribution of the deposits have been found in association with the functional and metabolic status of spermatozoa. The intracellular cytosol Ca++ concentration remains lower in comparison with the extracellular space, which results in disturbances of its regulation and the sensitivity to cold shock.
      • It has been found that activities of the enzymes LDH, NADH-diaphorase, alkaline and acidic phasphatases in spermatozoa of the muscus duck have been in correlation with the quality parameters of motility and concentration of spermatozoa. It has been demonstrated that sperm with high activity of LDH and NADH- diaphorase posseses higher fertilization capacity.
      • By employment of biochemical and microscopic techniques it has been shown that the cryopreservation of cattle sperm in the presence of the cryoprotectant GX results in: significant number of spermatozoa with injured acrosomal membrane; enhancement of the activity of alkaline phosphatase, acidic phosphatase and LDH; reduction of the activity of NADH-diaphorase and glucose-6-phosphate-dehydrogenase. Based on the obtained results prognostic evaluation of the fertilization capacity of cryopreserved cattle sperm by the use of the solvent GX has been made.
      • Comparative morphological and functional characterization of buffalo spermatozoa has been carried out in association with age, season and period of storage and cryopreservation. By employment of electron microscopy it has been found that the sperm solvents Triladil, Tris-Fructose, and Begly can cause reduction to a different degree of the activity of NADH-dehydrogenase, cytochrome reductase, cytochrome oxidase and LDH in spermatozoa, which is linked to a loss of membrane integrity and to a differentially manifested morphological injury.
      • The effect of Н2О2 in different concentrations on the vitality of spermatozoa in bulls, rams and boars has been examined. It has been found that cytochemically and electrophoretically determined activity of NADH, NADPH-diaphorase and LDH, has been influenced to different level in the particular species and depends on the concentration of the agent.
      • Line of research has been devoted to clarify the effects of hormonal and non-hormonal preparations (contraceptives) on the reproductive status and oogenesis of laboratory and farm animals.
      • Echography examinations of the reproductive apparatus in sheep and bulls during different seasons have been performed. Follicular waves have been observed, which are manifested during the oestrous season. Results may have relevance for the conduction of appropriate schedules of treatment for superovulation and synchronization
      • During morphological investigations of the ovarian dynamic and the folliculogenesis in bulls and sheep differences have been found, which have relevance to the embryotransplantation in these species.
      • The ultra-structure of male germinal cells of the water buffalo breed “Bulgarian Mura” and of the oocytes and embryos obtained after hormonal treatment for superovulation have been examined by means of scanning and transmission microscopy. Number of morphological characteristic features has been observed in the breed, as well as differences in the quality and quantity of germinal cells and embryos, obtained for embryotransfer.
      • Line of research has been devoted to examine the influence of hormonal treatment for the induction of superovulation on the properties of oocytes and embryos from sheep, goats and buffaloes. Based on the obtained results schedules of treatment have been made for the purposes of the embryotransplantation.
      • Team consisting of Bulgarian and Egyptian scientists (Principal Investigator on Bulgarian part Dr. D. Kacheva) has conducted successful embryotransfer and in 2005 and 2006 the first buffalo-calfs were born in Egypt. The results have been promising and give reason to continue the collaboration on buffalo embryotransfer and to expand studies on the technology in other species like sheep and goats. Under way are collaborated experiments for the induction of twin pregnancies in buffaloes aimed at enhancement of their reproductive capacity.
      • Schedules of hormonal synchronization of the oestrous cycle of the Greek milk-sheep breed Chios have been introduced and after cross-breeding with Bulgarian milk-sheep breeds the development in F1 has been examined. The follicular dynamic in the animals was studied by ultra-sonography and based on the findings the most appropriate time for insemination was chosen. Early diagnostic of pregnancy was introduced by means of echographic examinations of the inseminated animals. These studies have importance for the elaboration of approaches for maximum use of the natural reproductive capacity of the particular breeds.
      • It has been found that application of ethylene glycol as a cryoprotectant for the preservation of pre-implantation goat embryos ensures their successful long-term preservation without obvious aberrations in the morphology and vitality, which has been confirmed by the outcome of pregnancies after performed embryotransfer.
      • In progress are experiments to carry out embryotransfer of freshly obtained sheep and goats during the anoestrous cycle by the employment of laparoscopic and echographic methods.
      • Studies aimed at search of possibilities to achieve ecologically healthy animal production have revealed that the preparation Tribestan can exert positive effect on the sperm production of rams during the oestrous season. In New Zeeland rabbits the addition of optimum doses of Tribestan to the dietary regime has improved the ovarian dynamic of the animals.
      • Research grants

        1. 2К-11-03/06 Institute of Zoology, Academy of Sciences of China – Ministry of Education and Science. Improvement of the reproductive biotechnology embryotransfer in different species of farm animals.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        Project leader: Assoc. Prof. Dr. D. Kacheva.
        2. 2002-2004 Academy Exchange with the Chech Academy of Sciences. Receipt of buffalo embryos in vitro.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        3. 2002-2005 Academy of Scientific Research and Technology, Egypt. Embryotransfer in buffaloes.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        4. 2006-2008 Academy of Scientific Research and Technology, Egypt. Studies on the improvement of reproductive biotechnologies in farm animals.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        5. 2007-2010 Academic Exchange with Russian Scientific Research Institute of Physiology, Biochemistry and Nutrition; Russian Academy of Agriculture. Improvement of reproductive biotechnologies.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        6. 2007-2010 Latwian Veterinary Academy. Improvement of reproduction in domestic animals by employment of new biotechnologies.
        Project leader: Assoc. Prof. Dr. D. Kacheva.
        7. BG 2006/2008, Academic Exchange with the Institute of Animal Sciences, Komotini, Greece – Ministry of Education and Science. Studies on the F1 development in cross-breeds of Bulgarian milk sheep breed with the Greek milk sheep breed Chios.
        Project leaders: Assoc. Prof. E. Raycheva; Assoc. Prof. Dr. D. Kacheva.
        8. 4-01-2008/2009 “Balkan Network of biotechnologies in animal reproduction for the maintenance and rebuild of animal genetic resources in the region”, ASOs, Innovation Center, Vienna.
        Project leader from IBIR: Assoc. Prof. Dr. D. Kacheva.
        9. COST Action FA0602 Bioactive Food Components, Mitochondrial Function and Health (2007-2011 ) – supported within FP7.
        Project leaders: Prof. D.Kacheva, Assoc. Prof. Е.Kistanova
        10. БВ-5/2007. Investigation of approaches for higher effect of buffalo reproduction- Joint research project with Vietnam.
        Project leaders: Prof. D.Kacheva, Assoc. Prof. М.Kicheva
        11. BAS “Influence of the biological active substances on the female reproductive potential and embryo development” 2007-2011
        Project leader: Assoc. Prof. Е.Kistanova
        12. BAS “Morphological characteristic of sperm and spermatozoid-specific proteins and enzymes, related to fertilizing ability”
        Project leader: Assoc. Prof. B.Georgiev
        13. FP7-REGPOT-2009-1, GA 245641 (ReProForce) Reinforcement of the research capacity of the Bulgarian institute “Biology and Immunology of Reproduction” , 2009-2013 г.
        Scientific expert: Assoc. Prof. Е.Kistanova

        Scientific publications

        Scientific papers published during last 5 years.

        Papers in National (Bulgarian) scientific journals

        Abadjieva, D., Gospodinova, Ts., Stefanov, Rossen, Kistanova, E.   Morphological evaluation of gilt ovaries in relation to the quality of recovered oocytes. Bulgarian Journal of Veterinary Medicine, 18, 3, 209-215, 2015.

        Ст.Околийски, Б.Георгиев   Влиянието на електромагнитните полета и радиочестотната радиация върху репродукцията при хора. Андрология, 24, 1, 15-18, 2015.
        ISSN: 1013-3806

        Г.Йорданова, Д.Абаджиева, Р.Недева  Използване на Spirulina platensis при хранене на селскостопанските животни. Животновъдни науки, 51, 6, 67-72, 2014.
        ISSN: 0514-7441

        Д.Абаджиева   Храната на майката, определя бъдещето на детето. Вестник Технически авангард, бр. 2, стр. 13, 2014

        D.Abadjieva, M.Chervenkov, R.Stefanov, N.Metodiev, E.Kistanova, D.Kacheva, E.Raycheva   Effect of breeding season on the kinematic parameters and morphology of ram’ sperm from synthetic population Bulgarian milk sheep breed. Bulg.J.of Agricultural Sci., 20, 4, 967-972, 2014.
        ISSN 1310-0351

        Д. Качева   75 години Институт по биология и имунология на размножаването „Акад.К.Бртанов” – мисия за живот. Списание на БАН, 127, 1, 5-9, 2014.
        ISSN: 0007-3989

        Д.Качева   Ембриотехнологии при животните. Списание на БАН, 127, 1, 18-21, 2014.
        ISSN: 0007-3989

        R.Nedeva, G.Jordanova, E.Kistanova, K.Shumkov, B.Georgiev, D.Abadjieva, D.Kacheva, A.Shimkus, A.Shimkine   Effect of theaddition of Spirulina Platensis on the productivity and some blood parameters on growing pigs.  Bulg.J.of Agricultural Sci., 20, 3, 680-684, 2014.
        ISSN 1310-0351

        Р.Стефанов, Б.Георгиев, Д.Качева, П.Таушанова, Г.Ненкова, Н.Методиев, М.Червенков, А.Александрова   Морфофункционални характеристики и липидна пероксидация на сперматозоиди от вида ovisaries. Сборник доклади от научна конференция „Традиции и съвременност във ветеринарната медицина”, Лесотехнически университет, София, 2012 (отпечатано 2014).

        М.Червенков, Д.Даскалова, И.Кирилова, А.Колев, А.Куков   Изследване на основните спермални параметри на три застрашени от изчезване автохонни породи овце от западна България.   Сборник доклади от научна конференция „Традиции и съвременност във ветеринарната медицина”, Лесотехнически университет, София, 2012 (отпечатано 2014).

        M.Chervenkov,T.Ivanova, D.Kacheva, T.Stoeva   Antiviral activity of Melissa officinalis aqueous extract against pseudorabies virus. Compt.Rend.Acad.Bulg.Sci., 67, 6, 879-884, 2014.
        ISSN: 1310-1331       IF: 0.284

        Д. Чотински, Г. Михайлова, Д. Абаджиева, И. Денев   Ефект па xtract върху липидните компоненти в яйчния жълтък на кокошки носачки. Животновъдни науки, 50, 3, 30-40, 2013.
        ISSN: 0514-7441

        Д. Абаджиева, С. Григорова, Г. Вълчев, Е. Кистанова   Морфометрични параметри на яйчникови структури на зайци, приемали екстракт от Tribulus terrestris. Животновъдни науки, L, 6, 82-87. 2013.
        ISSN 0514-7441

        Д. Абаджиева, С. Григорова, Д. Качева, Е. Кистанова   Характеристика на преантрални фоликули на зайци от породата новозеландска бяла. Животновъдни науки, L, 4-5, 66-72, 2013.
        ISSN 0514-7441

        Ж.Накев, Е.Йорданова, Р.Недева, И.Марчев, Е.Кистанова, А.Шимкус, А.Шимкене, Е.Петров    Влияние на добавката от микроалги spirulina platensis върху кланичните качества и физикохимичния състав на muskulus longissimus dorsi при угоявани прасета. Животновъдни науки, L, 6, 10-15, 2013.
        ISSN 0514-7441

        A.Shimkus, A.Shimkiene, A.Stimbirys, N.Pechiulaitiene, L.B.Zavodnik, E.Kistanova, G.I.Boryaev, M.N.Nevitov, A.V.Ostapchuk   Impact of polyprobiotics on the productivity of broiler chickens. Животновъдни науки, L, 1, 32-39, 2013.
        ISSN 0514-7441

        Р.Стефанов, Е.Кистанова, Н.Методиев, Д.Качева, Е.Райчева, Б.Георгиев, П.Таушанова     Динамика в морфофункционалните параметри на сперматозоиди от коч от породата синтетична популация българска млечна в зависимост от сезона и индивидуалните им характеристики. Аграрни науки, 5, 13, 54-59, 2013.
        ISSN 1313-6577.

        R.Stefanov, D.Abadjieva, M.Chervenkov, E.Kistanova, D.Kacheva, P.Taushanova, B.Georgiev    Enzyme activities and motility of boar spermatozoa during 72-hour low temperature storage. Bulgarian Journal of Veterinary Medicine, 16, 4, 237-242, 2013.
        ISSN 1311-1477 (print); ISSN 1313-3543 (online).

        М.Червеиков, Т.Иванова, Д.Качева, Т.Стоева, Е.Кистанова   Влияние на растителни екстракти с антивирусни свойства върху преживяемостта на свински яйцеклетки. Животновъдни науки, 50, 3, 41-48, 2013.
        ISSN: 0514-7441

        К.Неделков, Н.Тодоров, Б.Георгиев, А.Атанасов   Увеличавае на плодовитта на овцете чрез фокусирано подхранване от 9-ия до 14-ия ден на половия цикъл, синхронизиран чрез ефекта на коча. Животновъдни науки, XLIX, стр.9-20, 2012.
        ISSN: 0514-7441

        B.Georgiev, S.Hayrabedyan, K.Todorova, D.Zasheva, P.Taushanova, D.Kacheva, P.J.Hansen   Sperm proteins as potential markers of boar fertility. Compt.Rendus Acad.Bulg.Sci., 65, 4, 533-540, 2012.
        ISSN 1310-1331. IF : 0.210

        Г.Йорданова, Р.Недева, Ж.Накев, Е.Кистанова, А.Шимкус, А.Шимкиене   Eфект от добавката на Spirulina platensis на продуктивността, някои кръвни показатели и процента постно месо при угоявани прасета. Животновъдни науки, XLIX, 6, 26-33, 2012.
        ISSN 0514-7441

        Р.Стефанов, Б.Георгиев, Д.Качева, П.Таушанова, М.Червенков, Е.Кистанова Изследване на LDH–Х и мотилитета при сперматозоиди от вида ovis aries.  Животновъдни  науки, XLIX, 6, 50-52, 2012.
        ISSN 0514-7441

        S.Hayrabedyan, B.Georgiev, D.Kacheva, M.Chervenkov, K.Shumkov, P.Taushanova, E.Kistanova   Flowcytometry as a Method for Advanced Evaluation of Boar Semen. Compt.Rendus Acad.Bulg.Sci., 65, 4,  541-549, 2012.
        ISSN 1310-1331. IF : 0.210

        Д.Чотински, Г.Михайлова, Д.Абаджиева, Е.Петков, И.Денев   Използване на полибактериален имуностимулатор (натстим) при отглеждане на кокошки носачки. ІІ. Липидни компоненти на яйчения жълтък. Животновъдни науки, XLVIII, 5, 2011.
        ISSN: 0514-7441

        N.Metodiev, D.Abadjieva, E.Raicheva, E.Kistanova   Study on some parameters of the consecutive ejaculates obtained from rams at various ages. 2nd Conference of the Balkan Network for the Biotechnology in Animal Reproduction. Proceeding, Sofia, 24-25 March 2011.
        ISSN: 1314-3085

        Т.Попова, Б.Георгиев, К.Генова, П.Кьосев, Й.Наков   Серологично проучване на разпространението на лептоспирозата сред домашните кучета в София през периода 2009-2010 г. Сборник доклади от научната конференция „Традиции и съвременност във ветеринарната медицина”, Лесотехнически университет, София, 2010, Издателска къща при ЛТУ, София, 2011.
        ISSN: 1313-4337

        Р.Стефанов, К.Лазов, М.Събев, К.Митева, Х.Христев   Виабилитет на спермазоиди от вида ovis aries след сепариране на спермалната плазма. Сборник доклади от научната конференция „Традиции и съвременност във ветеринарната медицина”, Лесотехнически университет, София, 2010, Издателска къща при ЛТУ, София, 2011.
        ISSN: 1313-4337

        R.Stefanov, M.Sabev, K.Miteva   Influence of the enzyme superoxide dismutase isolated from the fungal strain humicola lutea 103 on the content of carbonyl groups in the intracellular proteins upon in vitro cryopreservation of ram sperm. 2nd  Conference of the Balkan Network for the Biotechnology in Animal Reproduction. Proceeding, Sofia, 24-25 March 2011.
        ISSN: 1314-3085

        R.Stefanov, M.Sabev, M.Ivanova-Kicheva, A.Kukov, G.Anev, K.Lazov, K.Miteva   Studies on the fertility of sheep after artificial insemination with storied semen in hypothermic conditions. Compt.Rendus Bulg.Acad.Sci., 64, 5, 757-762, 2011.
        ISSN: 1310-1331; IF – 0.219

        М.Червенков, Д.Качева, Б.Георгиев, Е.Кистанова, К.Шумков   Санитарни аспекти на биотехнологията ембриотрансфер при животните – методи за пречистване на ооцити и ембриони. Сборник доклади от научната конференция „Традиции и съвременност във ветеринарната медицина”, Лесотехнически университет, София, 2010, Издателска къща при ЛТУ, София, 2011.
        ISSN: 1313-4337

        Chervenkov M., Shumkov K. Kistanova E. Kacheva D.   Procedures for disinfection of semen used in assisted reproductive techniques in animals. 2nd Conference of the Balkan Network for the Biotechnology in Animal Reproduction, Sofia, 24-25 March, 2011.
        ISSN 1314-308


        Papers in International peer-reviewed scientific journals

        Й.Марчев, Н.Павлова, Д.Абаджиева, М.Червенков, В.Младенова, Е.Кистанова   Возможности улучшения возпроизводства аборигенной породы Восточно-Балканская свинья в естественной среде обитания. Междунар.научно-практическая конф. „Современные аспекты воспроизводства сельскохозяйственных животных, Пенза, 15-16.05.2015, стр.3-11, Пенза, 2015.

        E.Kistanova, M.Chervenkov, K.Shumkov, R.Peshev, K.Todorova, S.Hayrabedyan, D.Abadjieva, A.Shimkus, A.Shimkiene   Immunostimulatory Properties of Spirulina platensis against Rabbit Hemorrhagic Disease. Pakistan Veterinary Journal, 35, 3, 379-381,  2015
        ISSN: 0253-8318       IF: 1.392

        R.Stefanov, G.Anev, D.Abadjieva  Effect of different extenders and storage periods on motility and fertility of ram sperm. Mac.Vet.Rev., 38, 1, 85-89, 2015.
        ISSN 1409-7621

        A.Simkiene, A.Simkus, S.Tusas, S.Makauskas, A.Stimbirys, G.Gerulis, E.Kistanova   Microalgae Spirulina platensis on dairy goat production. Wulfenia, 21, 1, 228-233, 2014
        ISSN: 1561-882X      IF: 1.312

        D. Abadjieva, M. Chervenkov, K. Shumkov, A. Shimkus, A. Shimkiene, E. Kistanova   Improving of productive and reproductive performances in rabbits by biologically active feed additives. Biotechnology in Animal Husbandry. Proceedings of the 10lh International Symposium Modem Trends in Livestock Production, Belgrade, Serbia, 2-4 October, 2013, p. 288-298.
        ISBN 978-86-82431-69-5.

        O.M.Kandil, A.S.S.Abdoon, D.Kacheva. B.Georgiev   Effect of In vitro Culture Media on Embryo Developmental Competence and Vitrification Methods in Blastocyst Viability in Buffalo. Global veterinaria, 11,5, 622-629, 2013.
        ISSN 1992-6197.

        R.Stefanov, M.Chervenkov, E.Kistanova, D.Kacheva, B.Georgiev, P.Taushanova, G.Nenkova, A.Aleksandrova    Lipid peroxidation and motility of drake spermatozoa after 24 hours of storag. Proceedings of the 10th International Symposium Modern Trends in Livestock Production, Belgrade, Serbia, 2-4 October, 2013,  p.847-853.
        ISBN 978-86-82431-69-5.

        Kandil O.M., A.S.S.Abdoon, D.Kacheva, C.H.Karaivanov, M.S.Fadel, N.A.Hemeida, B.Georgiev, T.I.Maslev, W.M.Ahmed, H.R. Badr   Successful Embryo Transfer in Egyptian Buffaloes. Global Veterinaria, 8, 4, 320- 327, 2012.
        ISSN 1992-6197.

        R.Stefanov, M.Chervenov, B.Georgiev, D.Kacheva, P.Taushanova, E.Kistanova, M.Sabev   Activity of nadh-tetrazolium reductase in bull semen after cryopreservation in relation to their motility. Book of Proceedings 3rd International Scientific Meeting “Days of Veterinary Medicine 2012”,  Skopje, Macedonia, 2-4 September 2012.

        Абаджиева Д. В., Кистанова Е. К.   Возможности стимулирования репродуктивных функций у самок животных (Литературный обзор). Нива Поволжья, 4 (21), 71-75, 2011.
        ISSN 1998-6092

        Абаджиева Д. В., Кистанова Е. К.   Современные аспекты стимулирования репродуктивных функций у самок животных. Образование, наука, прктика: иновационный аспект. Сборник  материалов международной научно-практической конфереции, посвященной 60 летию ФГБОУ ВПО „Пензенская ГСХА”, T. 2, 3-4, 2011.

        D.Abadjieva, K.Shumkov, E.Kistanova, D.Kacheva, B.Georgiev   Opportunities for the improvement of the reproductive performances in female animals. Biotechnology in Animal Husbandry, 27, 3, 365-372, 2011.
        ISSN 1450-9156

        Кравченко Ю., Г.Боряев, Ю.Петрунин, А.Баулин, E.Кистанова   Влияние экдистероидсодержащего сырья животного произхождения (трутневый разплод) на половое поведение крыс-самцов в онтогенезе. Образование, наука, прктика: иновационный аспект. Сборник  материалов международной научно-практической конфереции, посвященной 60 летию ФГБОУ ВПО „Пензенская ГСХА”, T.2, 21-22, 2011.

        R.Stefanov, M.Sabev, M.Ivanova-Kicheva   Protective effect of the antioxidant enzyme on sperm from Bubalus bubalis type after cryoconservation. Biotechnology in Animal Husbandry, 27, 3, 943-949, 2011.
        ISSN: 1450-9156

        Шумков К., Е.Кистанова. Контролирование репродуктивных процессов у овец через гипоталамо-гипофизарно-гонадальную ось. Образование, наука, прктика: иновационный аспект. Сборник  материалов международной научно-практической конфереции, посвященной 60 летию ФГБОУ ВПО „Пензенская ГСХА”, T.2, 6-8, 2011.

        Шумков K., К. Кистанова. Нoвые аспекты контролирования репродуктивных процессов у овец через  гипоталамо-гипофизарно-гонадальную ось. Нива Поволжья, No 2 (19), 2011.
        ISSN: 1998-6092


        Scientific publications 2005-2010

        Published in Bulgaria:

        1. Герзилов В. , Б. Георгиев, И. Николов, Д. Качева (2005). Зависимост между ключови ензими и показатели на спермопродукцията при мускусни патоци. В: Научни трудове, АУ Пловдив, 1, 3, 127-130.

        2. Качева Д., М. Петров, Ц. Маслев, Б. Георгиев, О. Кандил, А. Абдун, И. Ралчев, К. Влахов, Ц. Христова, Ж. Колева (2005). Относно репродуктивния потенциал за ембриотрансфер на овце в анестрален сезон. Сб. Научна конференция “10 години Факултет по ветеринарна медицина при Лесотехнически университет (1994-2004) ” , София, 150-155.

        3. М. Петров, Д. Качева, М. Петрова, И. Вангелов, И. Кръстева, Ст. Николов, М. Иванова, 2005. Проучване на фармакологичното действие на екстракти от Artemisia Annua L. Върху фоликулогенезата и овулацията при мишки. Сборник доклади от науч. конфер., ФВМ- ЛТУ, София, стр. 156-162.

        4. Maslev Ts., D. Kacheva, B. Georgiev, O. Kandil, A. Abdun, J. Koleva.( 2006 ) The embryo transplantation like opportunity for utilize of the reproductive potential of the sheep in seasonal anoestrus. Journal of Mountain Agriculture on the Balkans, 9, 5, 687-695.

        5. Георгиев Б., Е. Кистанова, Д. Качева, Е. Райчева, Е.Киприотис. Н. Методиев, Б. Григоров (2006). Ултрасонографско проучване на овариална реакция след синхронизация на еструса в размножителния сезон при български местни породи овце. Вет. Мед., год. X, № 3- 4, 46-51.

        6. Качева Д., О. Кандил (2006). Биотехнологични методи за подобряване продукцията на биволски ембриони in vitro. Сб. Научна конференция ”Традиции и съвременност във ветеринарната медицина”, София, 174-179.

        7. Rycheva D., D. Nedelchev, T. Ivanova, L. Kozelov, E. Kistanova, G. Dimov, D. Kacheva, E. Kipriotis, K. Kousenidis, T.Papadopoulos(2006). Study of the performances of the f1 crosses of the bulgarian dairy sheep breeds with the greek Chios dairy breed. I. Milk yield of the first sucking control of the Karakachan and Westbalkanmountain sheep and average daily gain of the purebred lambs and f1 crosses with Chios. Journal of Mountain Agriculture on the Balkans, 9, 1, 43-53.

        8. Качева, Д., (2007). Приложение на ембриобиотехнологиите в репродукцията на животните. Животновъдство, бр. 4, стр. 9-11.

        9. Kacheva D., B. Georgiev, J. Koleva, A. Abdoon, O. Kandil (2007). Effect of cryopreservation on mitochondrial activity in buffalo sperm Bubalus bubalis. Proc. of the 8-th World Buffalo Congress, Caserta, October 19-22, 2007, in Ital. J. Anim. Sci., 6, (Suppl.2), 769-771.

        10. Georgiev B., D. Kacheva, Tz. Peeva, E. Kistanova, E. K. Duan, Q.Y. Sun, D. Y. Chen (2007). Cytochemical determination of the mitochondrial specific enzymes in cryopreserved buffalo sperm. J. Mount. Agr. Balkans, 10, 5, 956-964.

        11. Кравченко Ю., Г.Боряев, М.Невитов, А.Остапчук, Е. Кистанова, Д. Качева. Протективен ефект на селенопиран в условията на оксидативния стрес, предизвикан от кадмиевия дихлорид. Сб. доклади от научна конференция в ФВМ, ЛТУ, 23 ноември 2007, София, стр.202-210.

        12. Dimitrov A., M. Ivanova-Kicheva, D. Daskalova, D. Kacheva, E. Solenkova, (2007). Protective role of separated seminal plasma proteins in in vitro storage of ram spermatozoa. J. Mount. Agr. Balkans, Vol. 10, 5, p. 922-932.

        13. Качева Д., Ц. Маслев, Е-К. Дуан, К-Ю.Сун, Д-Ю Чен, М.Петров, Е.Кистанова, Георгиев Б, Е.Сапунджиев, Ж.Колева, К.Шумков/2007/. Сравнение на възможностите на биотехнологичния метод МОЕТ за отключване на репродуктивния потенциал на овце в ранен и късен стадий на анестрален сезон. Сб. доклади от научна конференция в ФВМ, ЛТУ, 23 ноември 2007, София, стр.195-201.

        14. Кистанова E., Д.Качева, Е .Райчева Н. Методиев,Б.Георгиев, Е.Киприотис (2008). Полово развитие на F1 кръстоски на породата Xиос в условията на българия./ Sexual Development of the F1 Chios crosses in the conditions of Bulgaria. Journal of Mountain Agriculture on the Balkans (ISSN: 1311-0489), vol.11, 1, pp.21-30.

        15. Качева, Д., М. Кичева, Е, Кистанова, Ж. Колева, К. Шумков, 2008. Морфологични изследвания върху криоконсервирана сперма от воден бивол Bubalus Bubalis. Сборник доклади от научна конференция „Традиции и съвременост във ветеринарната медицина”, ЛТУ- София, стр.63-71.

        16. Даскалова Д., М. Иванова-Кичева, Л. Куков, Е. Соленкова, Д. Качева, М. Петров, Т. Маринков, 2008. Роля на фракционирани липопротеини от яйчен жълтък върху мотилитета и преживяемостта на сперматозоиди от коч при съхранение на 4С. Сборник доклади от научна конференция „Традиции и съвременост във ветеринарната медицина”, ЛТУ- София, стр.154-159.

        17. R. Nedeva, А. Аpostolov, У. Marchev, Е. Кistanova, D. Kacheva, К. Shumkov, G. Borjaev, (2009). Effect of the Injective Application of Selenopyran on the Productivity in Growing Gilts. Bulgarian Journal of Agricultural Science (ISSN: 1310-0351), V.15,№ 6, 604-609.

        18. Kistanova, Е., Dimitrina Kacheva, Kiril Shumkov, Desislava Abadjieva, Gennadiy Borjaev, Mihail Nevitov, Almantas Shimkus. Effect of biological active substances on the mouse embryo production in vivo. Доклади на Българската академия на науките, Comptes rendus de l’Acad´emie bulgare des Sciences (ISSN: 1310-1331), Tome 62, No 4, p. 499-506, 2009. IF: 0.204.

        Published abroad:

        1. Kistanova Е., H. Zlatev, V.Karcheva, A.Kolev /2005/ Effect of plant tribulus terrestris extract on reproductive performances of rams. Biotechnology in Animal husbandry, 21:1-2, pp.55-63.

        2. M.Mollova, M.Ivanova, B.Atanassov, M.Petrov, St. Kyurkchiev (2005). Study of the role of a sperm membrane-associated antigen in canine fertilization. Reproductive Biology, Vol.5, No.3, 331-339.

        3. Kistanova E./2005/ Improvement of the reproductive performances of rams by the biological active substances – plant extract and probiotic. Biotechnology in Animal husbandry.21:5-6, book 1, pp.68-72

        4. Raicheva Е., E. Kistanova /2005/ Individual laboratory yield of cheese from control day milk of Pleven Blackhead sheep breed. Biotechnology in Animal husbandry, 21:5-6,book 2,pp. 381-385.

        5. M. Mollova, B. Atanassov, R. Nedkova, S. Kyurkchiev (2006). Biochemical and
        immunochemical characterization of boar sperm flagellar protein with role in hyperactivation/capacitation process. Reproductive Biology, Vol.6, No. 1.

        6. Методиев Н., Б. Григоров, Б.Георгиев, Е.Кистанова, Д. Качева, Е.Райчева, (2006). Диагностические возможности ультразвукового метода при исследовании репродуктивных органов овец. Сборник межд. научн. конф. “Актуальные проблемы диагностики, терапии и профилактики болезней животных». 15-16 март, Воронеж , Русия , стp. 91-95.

        7. Kacheva D., O. Kandil, A. Abdoon, T. Maslev, E. Duan, Q. Y. Sun, D. Chen, M. Petrov, E. Kistanova, B. Georgiev, E. Sapundjiev, J. Koleva, K. Shumkov (2007). Comparison of the abilities of biotechnology MOET for unlocking of the ewes reproductive potential in different stages of seasonal anoestrus. 14-th International Conference on Biotechnology in Animal of Reproduction (ICBAR,2007), Cairo, Egypt, 62-63.

        8. Kandil O., A. Abdoon, D. Kacheva, Ch. Karaivanov, M. Fadel, N. Hameida, B. Georgiev, T. Maslev, W. Ahmed, H. Raffat (2007). The first Egyptian buffalo calf born through non-surgical embryo recovery and transfer. 14-th International Conference on Biotechnology in Animal of Reproduction (ICBAR,2007), Cairo, Egypt, 60-61.

        9. Kravchenko U., G.Borjaev, M. Nevitov, A.Ostapchuk, E.Kistanova /2007/The role of the antioxidant – selenopyran in the stability of the antioxidant system to toxic effec of cadmium chloride. Biotechnology in Animal husbandry, 23:5-6, book 1 pp. 181-191.

        10. Raycheva E., T.Ivanova, E. Kipriotis, E.Kistanova./2007/The caracteristic of control day milk and its properties in ewes from different breeds in Bulgaria. Biotechnology in Animal husbandry, 23:5-6, book 2 pp.139-144.

        11. Kistanova E., D.Vasileva, B.Grigorov,N.Metodiev./2007/ The morphological and biochemical characteristics of the consecutive ejaculates from Il-de-france rams at various ages. Biotechnology in Animal husbandry, 23:5-6, book 1 pp. pp.301-310.

        12. Кистанова Е., Колев А., Златев Х. /2007/ Влияние Трибестана на половое поведение и спермопродуктивность баранов в сезон размножения. Проблемы биологии продуктивных животных, №2, стр.75-79.

        13. Marchev Y, R. Nedeva, A. Apostolov, E. Kistanova, D. Kacheva, D. Adzhieva, J. Koleva, K. Shumkov, G. Boryaev, M. Nevitov (2008). Effect of Selenopyran treatment on the blood parameters and reproductive system of gilts. International Scientific Conference “The tropical problems of pigs and poultry nutrition. Quality production”. Proc. Intern. Sci. Conference, Kaunas, 78-84.

        14. Kacheva D., E. Kistanova, B. Georgiev, E. Raycheva, N. Metodiev, B. Grigorov, E. Kipriotis (2008). The study of the ovaries’ state in local Bulgarian ewe’s breeds by real-time ultrasonography. Biotechnology in Animal Husbandry (ISSN: 1450-9156), 24,:3-2, 57-65.

        15. Кистанова Е, Д. Качева, Ж. Колева, К. Шумков, Р. Недева, Й. Марчев, А. Аростолов, Д. Аджиева, Г. Боряев, М. Невитов, (2008). Развитие репродуктивной системы и интенсивность роста у ремонтных свиней, получавших селенопиран при выращивании от 30 до 100 кг живого веса. Сборник Международной н-пр. конференции “Образование, наука, практика:инновационный аспект” г.Пенза, Русия, стр. 434-435.

        16. Кистанова Е, Д. Качева, Г.Вылчев, А.Иванов, Ж.Колева,К.Шумков, (2008). Доза-зависимый эффект екстракта Тribulus terrestris l. на фолликулярную активность яичников крольчих новозенландской породы. Сборник Международной н-пр. конференции “Образование, наука, практика: инновационный аспект”, г.Пенза, Русия, стр. 432-434.

        17. Боряев Г., Ю.Каравченко., Е.Кистанова.,М.Невитов., А.Остапчук /2008/. Устойчивость ферментного звена антиоксидантной системы органов и тканей крыс к токсическому воздействию на этапах онтогенеза. Нива Поволжья, №4 /9/, стр. 68-71.

        18. Kistanova, Е., Y. Marchev, R. Nedeva , D. Kacheva, K. Shumkov, B. Georgiev, A. Shimkus, (2009). Effect of Spirulina platensis included in the main diet on the boar sperm quality. Biotechnology in Animal Husbandry (ISSN: 1450-9156), 25 (5-6), Book 1, p. 547-557.

        Scientific-popular publications and books:

        1. Кънчев Л., Ж. Байчев, Д. Качева. Асистирани репродуктивни технологии в животновъдството. София, изд. „Виденов и син”, 233 стр., 2010.

        1. Качева Д. Трансплантация на ембриони при животни в България. Новости, БАН, Сборник с постижения на учени от БАН, бр. 12, 59, 2005.
        2. Кистанова Е.”Качеството на мъжката сперма спадна драстично”, Интервю за българския вестник “Доктор”, 28-5 юли, 2010 .